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Indirect and competitive enzyme-linked immunosorbent assays for monitoring the humoral response against human VEGF.
Sánchez Ramírez, Javier; Morera Díaz, Yanelys; Musacchio Lasa, Alexis; Bequet-Romero, Mónica; Muñoz Pozo, Yasmiana; Pérez Sánchez, Lincidio; Hernández-Bernal, Francisco; Mendoza Fuentes, Osmany; Selman-Housein, Katty-Hind; Gavilondo Cowley, Jorge Víctor; Ayala Avila, Marta.
Afiliação
  • Sánchez Ramírez J; a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Morera Díaz Y; a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Musacchio Lasa A; b Department of Bioinformatics , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Bequet-Romero M; a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Muñoz Pozo Y; a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Pérez Sánchez L; a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Hernández-Bernal F; c Department of Clinical Research , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Mendoza Fuentes O; d Animal Facility , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Selman-Housein KH; e Center of Medical and Surgical Research (CIMEQ) , Playa Siboney, Havana , Cuba.
  • Gavilondo Cowley JV; a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
  • Ayala Avila M; a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
J Immunoassay Immunochem ; 37(6): 636-58, 2016.
Article em En | MEDLINE | ID: mdl-27143151
CIGB-247, a VEGF-based vaccine, was studied in a clinical trial. This advance demands the refinement of the methodologies for assessment of vaccine immune responses. This study aimed to improve the performance of ELISAs for detecting IgG antibodies against human VEGF and the blocking activity of the serum to inhibit the VEGF/VEGFR2 interaction. The best experimental conditions were established through the evaluation of several blocking buffers, immobilization surfaces, and plate suppliers using human sera as test samples. As a result, two controlled ELISAs were used in testing of elicited immune response against VEGF in patients immunized with CIGB-247.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Vacinas Anticâncer / Receptor 2 de Fatores de Crescimento do Endotélio Vascular / Fatores de Crescimento do Endotélio Vascular / Imunidade Humoral Tipo de estudo: Clinical_trials Limite: Animals / Humans Idioma: En Revista: J Immunoassay Immunochem Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Cuba País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Vacinas Anticâncer / Receptor 2 de Fatores de Crescimento do Endotélio Vascular / Fatores de Crescimento do Endotélio Vascular / Imunidade Humoral Tipo de estudo: Clinical_trials Limite: Animals / Humans Idioma: En Revista: J Immunoassay Immunochem Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Cuba País de publicação: Reino Unido