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Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli.
Gerner, Lisa; Munack, Steffi; Temmerman, Koen; Lawrence-Dörner, Ann-Marie; Besir, Hüseyin; Wilmanns, Matthias; Jensen, Jan Kristian; Thiede, Bernd; Mills, Ian G; Morth, Jens Preben.
Afiliação
  • Gerner L; Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway.
  • Munack S; Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway.
  • Temmerman K; European Molecular Biology Laboratory Hamburg, Notkestrasse 85, 22603 Hamburg, Germany; European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
  • Lawrence-Dörner AM; European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany; Institute of Biochemistry, WWU Münster, Wilhelm-Klemm-Straße 2, 48149 Münster, Germany.
  • Besir H; European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
  • Wilmanns M; European Molecular Biology Laboratory Hamburg, Notkestrasse 85, 22603 Hamburg, Germany.
  • Jensen JK; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
  • Thiede B; Department of Biosciences, University of Oslo, Norway; Biotechnology Centre of Oslo, University of Oslo, Norway.
  • Mills IG; Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway; Department of Molecular Oncology, Institute for Cancer Research, Oslo University Hospitals, Norway; Movember/Prostate Cancer UK Centre of Excel
  • Morth JP; Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway; Institute for Experimental Medical Research, Oslo University Hospital, Norway.
Data Brief ; 8: 733-40, 2016 Sep.
Article em En | MEDLINE | ID: mdl-27508226
Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 'apo', CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required and the proteins subsequently used to develop a fluorescence-based assay for drug binding to the kinase, "Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2" [1].
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Data Brief Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Noruega País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Data Brief Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Noruega País de publicação: Holanda