Analysis of mitochondrial genetic diversity of Ustilago maydis in Mexico.
Mitochondrial DNA A DNA Mapp Seq Anal
; 29(1): 1-8, 2018 01.
Article
em En
| MEDLINE
| ID: mdl-27728988
The current understanding of the genetic diversity of the phytopathogenic fungus Ustilago maydis is limited. To determine the genetic diversity and structure of U. maydis, 48 fungal isolates were analyzed using mitochondrial simple sequence repeats (SSRs). Tumours (corn smut or 'huitlacoche') were collected from different Mexican states with diverse environmental conditions. Using bioinformatic tools, five microsatellites were identified within intergenic regions of the U. maydis mitochondrial genome. SSRMUM4 was the most polymorphic marker. The most common repeats were hexanucleotides. A total of 12 allelic variants were identified, with a mean of 2.4 alleles per locus. An estimate of the genetic diversity using analysis of molecular variance (AMOVA) revealed that the highest variance component is within states (84%), with moderate genetic differentiation between states (16%) (FST = 0.158). A dendrogram generated using the unweighted paired-grouping method with arithmetic averages (UPGMA) and the Bayesian analysis of population structure grouped the U. maydis isolates into two subgroups (K = 2) based on their shared SSRs.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ustilago
/
Variação Genética
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Repetições de Microssatélites
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Genoma Mitocondrial
País/Região como assunto:
Mexico
Idioma:
En
Revista:
Mitochondrial DNA A DNA Mapp Seq Anal
Ano de publicação:
2018
Tipo de documento:
Article
País de publicação:
Reino Unido