Your browser doesn't support javascript.
loading
Bovine in vitro embryo production: the effects of fibroblast growth factor 10 (FGF10).
Diógenes, Mateus Nunes; Guimarães, Ana Luiza Silva; Leme, Ligiane Oliveira; Dode, Margot Alves Nunes.
Afiliação
  • Diógenes MN; School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.
  • Guimarães AL; School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.
  • Leme LO; Embrapa- Genetic Resources and Biotechnology, Laboratory of Animal Reproduction, Parque Estação Biológica, Final Av. W5/N, Prédio, PBI70770-900, Brasília, DF, Brazil.
  • Dode MA; School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.
J Assist Reprod Genet ; 34(3): 383-390, 2017 Mar.
Article em En | MEDLINE | ID: mdl-28000057
PURPOSE: In an attempt to improve in vitro embryo production, we investigated the effect of fibroblast growth factor 10 (FGF10) during in vitro maturation on the developmental capacity of bovine oocytes. MATERIAL AND METHODS: Cumulus-oocyte complexes (COCs) were aspirated from follicles of 3-8 mm diameter. After selection, the COCs were matured in medium with or without 0.5 ng/mL of FGF10. The effect of FGF10 during in vitro maturation (IVM) on nuclear maturation kinetics and expansion of the cumulus cells was investigated. Oocyte competence was assessed by the production and development speed of embryos and the relative expression of genes associated with embryo quality. RESULTS: FGF10 delayed the resumption of meiosis from 8 h onwards, but did not affect the percentage of oocytes reaching metaphase II, nor did it increase cumulus expansion at 22 h of maturation. We found no difference between treatments regarding embryo production, developmental speed, and gene expression. CONCLUSION: In conclusion, the presence of FGF10 during IVM had no effect on embryo production, developmental speed, and gene expression.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Desenvolvimento Embrionário / Fator 10 de Crescimento de Fibroblastos / Técnicas de Maturação in Vitro de Oócitos Limite: Animals Idioma: En Revista: J Assist Reprod Genet Assunto da revista: GENETICA / MEDICINA REPRODUTIVA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Desenvolvimento Embrionário / Fator 10 de Crescimento de Fibroblastos / Técnicas de Maturação in Vitro de Oócitos Limite: Animals Idioma: En Revista: J Assist Reprod Genet Assunto da revista: GENETICA / MEDICINA REPRODUTIVA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda