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Three-Dimensional Reconstruction of the Tissue-Specific Multielemental Distribution within Ceriodaphnia dubia via Multimodal Registration Using Laser Ablation ICP-Mass Spectrometry and X-ray Spectroscopic Techniques.
Van Malderen, Stijn J M; Laforce, Brecht; Van Acker, Thibaut; Nys, Charlotte; De Rijcke, Maarten; de Rycke, Riet; De Bruyne, Michiel; Boone, Matthieu N; De Schamphelaere, Karel; Borovinskaya, Olga; De Samber, Björn; Vincze, Laszlo; Vanhaecke, Frank.
Afiliação
  • Van Malderen SJ; Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
  • Laforce B; Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
  • Van Acker T; Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
  • Nys C; Department of Applied Ecology and Environmental Biology, Ghent University , Jozef Plateaustraat 22, 9000 Ghent, Belgium.
  • De Rijcke M; Department of Applied Ecology and Environmental Biology, Ghent University , Jozef Plateaustraat 22, 9000 Ghent, Belgium.
  • Boone MN; Department of Physics and Astronomy, Ghent University , Proeftuinstraat 86, 9000 Ghent, Belgium.
  • De Schamphelaere K; Department of Applied Ecology and Environmental Biology, Ghent University , Jozef Plateaustraat 22, 9000 Ghent, Belgium.
  • Borovinskaya O; TOFWERK AG , Uttigenstrasse 22, 3600 Thun, Switzerland.
  • De Samber B; Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
  • Vincze L; Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
  • Vanhaecke F; Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
Anal Chem ; 89(7): 4161-4168, 2017 04 04.
Article em En | MEDLINE | ID: mdl-28256828
In this work, the three-dimensional elemental distribution profile within the freshwater crustacean Ceriodaphnia dubia was constructed at a spatial resolution down to 5 µm via a data fusion approach employing state-of-the-art laser ablation-inductively coupled plasma-time-of-flight mass spectrometry (LA-ICP-TOFMS) and laboratory-based absorption microcomputed tomography (µ-CT). C. dubia was exposed to elevated Cu, Ni, and Zn concentrations, chemically fixed, dehydrated, stained, and embedded, prior to µ-CT analysis. Subsequently, the sample was cut into 5 µm thin sections that were subjected to LA-ICP-TOFMS imaging. Multimodal image registration was performed to spatially align the 2D LA-ICP-TOFMS images relative to the corresponding slices of the 3D µ-CT reconstruction. Mass channels corresponding to the isotopes of a single element were merged to improve the signal-to-noise ratios within the elemental images. In order to aid the visual interpretation of the data, LA-ICP-TOFMS data were projected onto the µ-CT voxels representing tissue. Additionally, the image resolution and elemental sensitivity were compared to those obtained with synchrotron radiation based 3D confocal µ-X-ray fluorescence imaging upon a chemically fixed and air-dried C. dubia specimen.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imageamento Tridimensional / Imagem Multimodal Limite: Animals Idioma: En Revista: Anal Chem Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Bélgica País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imageamento Tridimensional / Imagem Multimodal Limite: Animals Idioma: En Revista: Anal Chem Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Bélgica País de publicação: Estados Unidos