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1,5-Diaminopentane production from xylooligosaccharides using metabolically engineered Corynebacterium glutamicum displaying beta-xylosidase on the cell surface.
Imao, Kenta; Konishi, Rie; Kishida, Mayumi; Hirata, Yuuki; Segawa, Shota; Adachi, Noriko; Matsuura, Rena; Tsuge, Yota; Matsumoto, Takuya; Tanaka, Tsutomu; Kondo, Akihiko.
Afiliação
  • Imao K; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Konishi R; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Kishida M; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Hirata Y; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Segawa S; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Adachi N; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Matsuura R; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Tsuge Y; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Matsumoto T; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Tanaka T; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
  • Kondo A; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan. Electronic address: akondo@kobe-u.ac.jp.
Bioresour Technol ; 245(Pt B): 1684-1691, 2017 Dec.
Article em En | MEDLINE | ID: mdl-28599919
Xylooligosaccharide-assimilating Corynebacterium glutamicum strains were constructed using metabolic engineering and cell surface display techniques. First, C. glutamicum was metabolically engineered to create lysine-producing strains. Beta-xylosidase BSU17580 derived from Bacillus subtilis was then expressed on the C. glutamicum cell surface using PorH anchor protein, and enzymes involved in the xylose assimilation pathway were also expressed. Metabolic engineering had no effect on the activity of beta-xylosidase. The engineered strains efficiently consumed xylooligosaccharides and produced 12.4mM of lysine from 11.9g/L of xylooligosaccharides as the carbon source. Finally, co-expression of lysine decarboxylase enabled production of 11.6mM of 1,5-diaminopentane (cadaverine) from 13g/L of consumed xylooligosaccharides.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Xilosidases / Corynebacterium glutamicum / Engenharia Metabólica Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Xilosidases / Corynebacterium glutamicum / Engenharia Metabólica Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido