Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-ß/ERK/p90RSK Signalling.
Stem Cells Int
; 2017: 6193106, 2017.
Article
em En
| MEDLINE
| ID: mdl-28928774
Y-box protein-1 (YB-1) is a highly conserved transcription factor that is involved in multiple biological processes via transcriptional regulation of several genes, including p53, cyclin D1, and EGFR. YB-1 has been reported to be overexpressed in injured livers. This study aims to explore the functions of YB-1 in hepatic progenitor cells (HPCs). Herein, chromatin immunoprecipitation sequencing (ChIP-sequencing) and RNA-sequencing assays identified that YB-1 participated in the biological adhesion process and ECM-receptor interactions in HPCs. Further study demonstrated that YB-1 modulated the expression of extracellular matrix components in HPCs. ChIP-sequencing assays established that PDGFR-ß was a target gene of YB-1, and luciferase reporter assays confirmed that YB-1 negatively regulated PDGFR-ß promoter activity in HPCs. In addition, PDGFR-ß can regulate the expression of collagen I through ERK/p90RSK signalling, and disruption of the signalling pathway with a PDGFR-ß inhibitor or ERK1/2 inhibitor abolished the regulatory effect of PDGFR-ß on collagen I expression in HPCs. Conclusively, YB-1 can modulate the expression of collagen I in HPCs via direct binding to the PDGFR-ß promoter, negatively regulating its expression. In addition, the ERK/p90RSK axis serves as the downstream signalling pathway of PDGFR-ß.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Stem Cells Int
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
Estados Unidos