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Comparison of Kato-Katz Thick Smear, Mini-FLOTAC, and Flukefinder for the Detection and Quantification of Fasciola hepatica Eggs in Artificially Spiked Human Stool.
Zárate-Rendón, Daniel A; Vlaminck, Johnny; Levecke, Bruno; Briones-Montero, Andrea; Geldhof, Peter.
Afiliação
  • Zárate-Rendón DA; Laboratorio de Parasitología, Facultad de Zootecnia, Universiad Nacional Agraria La Molina, Lima, Peru.
  • Vlaminck J; Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
  • Levecke B; Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
  • Briones-Montero A; Laboratorio de Parasitología, Facultad de Zootecnia, Universiad Nacional Agraria La Molina, Lima, Peru.
  • Geldhof P; Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
Am J Trop Med Hyg ; 101(1): 59-61, 2019 07.
Article em En | MEDLINE | ID: mdl-31074405
We compared the diagnostic performance of the standard method (Kato-Katz) with two recently developed methods (Mini-FLOTAC and Flukefinder) for the detection and quantification of Fasciola hepatica eggs in human stool. Uninfected human stool samples were artificially spiked with F. hepatica eggs to reach final concentrations of 14, 28, 41, or 96 eggs per gram of stool (epg). Only Flukefinder showed 100% sensitivity in all but the samples with the lowest concentration of eggs (14 epg), in which it had a sensitivity of 60%. Each of the methods underestimated the true fecal egg counts (FECs), Flukefinder resulting in the most biased egg counts (egg counts 0.18 times lower than the expected FECs). Only the Flukefinder resulted in more precise results (coefficient of variance < 30%) from FECs of 96 epg onward. The outcome of this study indicates that the Flukefinder is a useful alternative diagnostic method for human fascioliasis in stool.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óvulo / Contagem de Ovos de Parasitas / Fasciola hepatica / Fezes / Helmintíase Tipo de estudo: Diagnostic_studies Limite: Animals / Humans Idioma: En Revista: Am J Trop Med Hyg Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Peru País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óvulo / Contagem de Ovos de Parasitas / Fasciola hepatica / Fezes / Helmintíase Tipo de estudo: Diagnostic_studies Limite: Animals / Humans Idioma: En Revista: Am J Trop Med Hyg Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Peru País de publicação: Estados Unidos