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Using Differential Threshold Effects of Individual and Combined Periconceptional Methyl Donor Status on Maternal Genomic LINE-1 and Imprinted H19 DNA Methylation to Predict Birth Weight Variance in the Taiwan Pregnancy-Newborn Epigenetics (TPNE) Cohort Study.
Huang, Kuang-Ta; Shen, Yu-Li; Lee, Chien-Nan; Chu, Kuan-Yu; Ku, Wei-Chi; Liu, Chieh-Yu; Huang, Rwei-Fen S.
Afiliação
  • Huang KT; PhD Program in Nutrition and Food Science, Fu Jen Catholic University, New Taipei City, Taiwan.
  • Shen YL; Loving Care Maternity and Children's Health Centers, New Taipei City, Taiwan.
  • Lee CN; Department of Nutritional Science, Fu Jen Catholic University, New Taipei City, Taiwan.
  • Chu KY; Department of Gynecology and Obstetrics, National Taiwan University Hospital, Taipei City, Taiwan.
  • Ku WC; Department of Nutritional Science, Fu Jen Catholic University, New Taipei City, Taiwan.
  • Liu CY; School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
  • Huang RS; Biostatistical Consultant Lab, Department of Speech Language Pathology and Audiology, National Taipei University of Nursing and Health Sciences, Taipei City, Taiwan.
J Nutr ; 150(1): 108-117, 2020 01 01.
Article em En | MEDLINE | ID: mdl-31504733
BACKGROUND: Few studies have comprehensively examined the effect of methyl donor status on maternal DNA methylation and birth outcomes. OBJECTIVES: This study examined associations between periconceptional methyl donor status and genome-wide and specific imprinted gene methylation and fetal growth indices in the Taiwan Pregnancy-Newborn Epigenetics cohort. METHODS: Plasma folate, choline (free form), and betaine concentrations of the participants enrolled at 7-10 weeks of gestation were analyzed. DNA methylation at regulatory sequences of the imprinted H19 gene and genomic long interspersed nuclear element 1 (LINE-1) were measured in maternal lymphocytes using bisulfite/high-resolution melt polymerase chain reaction. Associations with birth weight (BW) were estimated through multiple regressions from 112 mother-newborn pairs. RESULTS: A nonlinear "L-shaped" relation and an inverse association between maternal plasma folate in T1 (mean ± SE: 17.6 ± 5.1 nmol/L) and lymphocytic LINE-1 methylation (ß: -0.49, P = 0.027) were characterized. After adjusting for LINE-1 methylation, individual maternal folate concentrations were positively associated with BW variance (ß = 0.24, P = 0.035), and the association was more pronounced in mothers with choline in T1 (mean ± SE: 5.4 ± 0.6 µmol/L; ß: 0.40, P = 0.039). Choline status of the mothers in T2 (mean ± SE: 7.2 ± 0.6 µmol/L) was inversely associated with LINE-1 methylation (ß: -0.43, P = 0.035), and a positive association was evident between T1 choline and H19 methylation (ß: 0.48, P = 0.011). After adjusting for epigenetic modification, maternal choline status predicted a positive association with BW (ß: 0.56, P = 0.005), but the effect was limited to mothers with high betaine concentrations in T3 (mean ± SE: 36.4 ± 8.8 µmol/L), depending on folate status. CONCLUSIONS: Our data highlight the differential threshold effects of periconceptional folate, choline, and betaine status on genomic LINE-1 and H19 DNA methylation and how their interplay has a long-term effect on BW variance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peso ao Nascer / Elementos Nucleotídeos Longos e Dispersos / Genômica / Epigenômica Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Newborn / Pregnancy País/Região como assunto: Asia Idioma: En Revista: J Nutr Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Taiwan País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peso ao Nascer / Elementos Nucleotídeos Longos e Dispersos / Genômica / Epigenômica Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Newborn / Pregnancy País/Região como assunto: Asia Idioma: En Revista: J Nutr Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Taiwan País de publicação: Estados Unidos