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Determination of minimum bactericidal concentration, in single or combination drugs, against Mycobacterium tuberculosis.
Santos, Nathally C de S; Scodro, Regiane B de L; Leal, Dayane Cb; do Prado, Silvia Mt; Micheletti, Daniela F; Sampiron, Eloísa G; Costacurta, Giovana F; de Almeida, Aryadne L; da Silva, Liliani Af; Ieque, Andressa L; Ghiraldi Lopes, Luciana D; de Pádua, Rubia Af; Siqueira, Vera Ld; Caleffi-Ferracioli, Katiany R; Cardoso, Rosilene F.
Afiliação
  • Santos NCS; Postgraduate Program in Bioscience and Physiopathology, State University of Maringá, Paraná, Brazil.
  • Scodro RBL; Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
  • Leal DC; Postgraduate Program in Health Sciences, State University of Maringá, Paraná, Brazil.
  • do Prado SM; Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
  • Micheletti DF; Postgraduate Program in Health Sciences, State University of Maringá, Paraná, Brazil.
  • Sampiron EG; Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
  • Costacurta GF; Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
  • de Almeida AL; Postgraduate Program in Health Sciences, State University of Maringá, Paraná, Brazil.
  • da Silva LA; Postgraduate Program in Health Sciences, State University of Maringá, Paraná, Brazil.
  • Ieque AL; Postgraduate Program in Bioscience and Physiopathology, State University of Maringá, Paraná, Brazil.
  • Ghiraldi Lopes LD; Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
  • de Pádua RA; Postgraduate Program in Health Sciences, State University of Maringá, Paraná, Brazil.
  • Siqueira VL; Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
  • Caleffi-Ferracioli KR; Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
  • Cardoso RF; Postgraduate Program in Bioscience and Physiopathology, State University of Maringá, Paraná, Brazil.
Future Microbiol ; 15: 107-114, 2020 01.
Article em En | MEDLINE | ID: mdl-32064924
Aim: To evaluate an assay to detect minimum bactericidal concentration (MBC) in Mycobacterium tuberculosis, using as single model rifampicin, isoniazid, levofloxacin (LVX) and linezolid (LNZ) and in combination. Material & methods: MBCs were carried out directly from resazurin microtiter assay plate and 3D checkerboard in M. tuberculosis H37Rv and five resistant clinical isolates. Results: The proposed MBC assay showed similar values to those determined by MGIT™, used as control. LVX and LNZ's MBC values were close to their MIC values. LNZ or LVX combined with isoniazid and rifampicin showed MBC value reduced in 63.7% of the assays. Conclusion: The proposed assay to determine MBCs of drugs can be applied to the study of new compounds with anti-M. tuberculosis activity to detect their bactericidal effect and also in laboratory routine for clinical dose adjustment of drugs according to the patient's profile.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Mycobacterium tuberculosis / Antituberculosos Limite: Humans Idioma: En Revista: Future Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Mycobacterium tuberculosis / Antituberculosos Limite: Humans Idioma: En Revista: Future Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido