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Generation and characterization of three induced pluripotent stem cell lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a 7-year-old healthy Caucasian male.
Ge, Ning; Liu, Min; Zhu, Xiaoran; Krawczyk, Janusz; McInerney, Veronica; Shen, Sanbing; O'Brien, Timothy; Prendiville, Terence.
Afiliação
  • Ge N; Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland.
  • Liu M; Department of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, China.
  • Zhu X; Department of Pharmacy, Hebei General Hospital, Shijiazhuang, China.
  • Krawczyk J; Department of Haematology, Galway University Hospital, Ireland.
  • McInerney V; HRB Clinical Research Facility, National University of Ireland (NUI) Galway, Ireland.
  • Shen S; Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland. Electronic address: Sanbing.Shen@nuigalway.ie.
  • O'Brien T; Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland. Electronic address: Timothy.OBrien@nuigalway.ie.
  • Prendiville T; Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland; National Children's Research Centre, Children's Health Ireland at Crumlin, Dublin 12, Ireland. Electronic address: Terence.Prendiville@olchc.ie.
Stem Cell Res ; 53: 102389, 2021 05.
Article em En | MEDLINE | ID: mdl-34088016
We report the generation of three human induced pluripotent stem cell (hiPSC) lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a healthy 7-year-old boy using non-integrational Sendai re-programming method expressing OCT4, SOX2, KLF4 and C-MYC. Stem cell characterization was confirmed through morphology, immunofluorescence staining and RT-qPCR. Differentiation potential in vitro was demonstrated to all three germ layers with STR lineage verification and normal molecular karyotyping through the process of re-programming.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas Limite: Child / Humans / Male Idioma: En Revista: Stem Cell Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Irlanda País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas Limite: Child / Humans / Male Idioma: En Revista: Stem Cell Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Irlanda País de publicação: Reino Unido