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Multilocus sequence typing of multidrug-resistant Salmonella strains circulating in poultry farms of Bangladesh.
Sultana, Khandokar Fahmida; Saha, Otun; Hoque, M Nazmul; Sultana, Munawar; Hossain, M Anwar.
Afiliação
  • Sultana KF; Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
  • Saha O; Department of Microbiology, Noakhali Science and Technology University, Noakhali, Bangladesh.
  • Hoque MN; Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
  • Sultana M; Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
  • Hossain MA; Department of Gynecology, Obstetrics and Reproductive Health, Bangabandhu Sheikh Muibur Rahman Agricultural University, Gazipur, 1706, Bangladesh.
Braz J Microbiol ; 52(4): 2385-2399, 2021 Dec.
Article em En | MEDLINE | ID: mdl-34297327
Salmonella is one of the most important foodborne zoonotic pathogens, and becoming multidrug-resistant (MDR), which represents a serious public health concern worldwide. This study aimed to identify the circulating MDR strains of Salmonella through cutting edge molecular techniques including gene specific PCR, RAPD-PCR, ribosomal gene sequencing, and multilocus sequence types (MLST) in the poultry industry of Bangladesh. Two hundred Salmonella isolates were retrieved from 154 samples comprising droppings (n = 60), cloacal swabs (n = 60), feeds (n = 14), feeding water (n = 14), and handler's swab (n = 6) from 14 commercial layer farms of Bangladesh. The isolates were confirmed as Salmonella through invA gene specific PCR, and further genotyping was done by RAPD-PCR, and 16S rRNA sequencing. The isolates were distributed into 18 different genotypes according to RAPD typing. The phylogenetic analysis identified three diverging phylogroups such as S. enterica Litchfield, S. enterica Enteritidis and S. enterica Kentucky with 11, 8, and 6 strains, respectively. The in vitro antibiogram profiling the Salmonella isolates through disc diffusion method using 13 commercially available antibiotics revealed highest resistance against doxycycline (91.5%) followed by tetracycline and ampicillin (86.0%, in each), and 72.0% isolates as MDR, being resistant to ≥ 5 antibiotics. The MLST typing was carried out based on the PCR amplification of seven housekeeping genes (aroC, hisD, hemD, purE, secA, thrA, and dnaN). MLST typing also revealed three sequence types (STs) such as ST11, ST198, and ST214 in these isolates, and eBURST analysis showed ST11 as the founder genotype. The three STs were highly resistant to tetracyclines and quinolone group of antibiotics, and all of the isolates harboring S. enterica Litchfield showed the highest resistance. Circulating common MLSTs with MDR properties in different farms confirmed the possibility of a common route of intra-farm transmission. We report for the first time of the association serovar Litchfield (ST11) in avian salmonellosis with MDR properties which is an urgent public health concern in Bangladesh.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aves Domésticas / Doenças das Aves Domésticas / Salmonella / Infecções por Salmonella / Resistência a Múltiplos Medicamentos / Tipagem de Sequências Multilocus / Antibacterianos Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Braz J Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Bangladesh País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aves Domésticas / Doenças das Aves Domésticas / Salmonella / Infecções por Salmonella / Resistência a Múltiplos Medicamentos / Tipagem de Sequências Multilocus / Antibacterianos Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Braz J Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Bangladesh País de publicação: Brasil