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Fabrication and characterization of Chinese yam polysaccharides PLGA nanoparticles stabilized Pickering emulsion as an efficient adjuvant.
Zhang, Yue; Jiao, Lina; Wu, Zhiyong; Gu, Pengfei; Feng, Zian; Xu, Shuwen; Liu, Zhenguang; Yang, Yang; Wang, Deyun.
Afiliação
  • Zhang Y; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
  • Jiao L; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
  • Wu Z; Nanjing Traditional Chinese Veterinary Medicine Research Center, Nanjing 210095, PR China.
  • Gu P; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
  • Feng Z; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
  • Xu S; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
  • Liu Z; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
  • Yang Y; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
  • Wang D; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: dywang@njau.edu
Int J Biol Macromol ; 209(Pt A): 513-524, 2022 Jun 01.
Article em En | MEDLINE | ID: mdl-35421409
The Chinese yam polysaccharides PLGA nanoparticles were applied as stabilizers in this study to prepare O/W Pickering emulsion. The optimized preparation conditions were PLGA concentration of 5 mg/mL, ultrasonic power of 50 %, and ultrasonic time of 2 min. The CYP-PPAS emulsion exhibits a raspberry-like morphology with a large number of nanoparticles surrounding the oil droplets. The CYP-PPAS emulsion exhibited outstanding stability at 4 °C and 37 °C for 28 days with high antigen loading efficiency and provided a controlled and sustained release of Chinese yam polysaccharides and OVA antigen in vitro. CYP-PPAS/OVA elicited robust antigen-specific immune response and induced a mixed Th1/Th2 immune response after immunization. Furthermore, CYP-PPAS/OVA caused a high CD4+/CD8+ ratio leading in increased activation of splenic T lymphocytes subpopulations. Moreover, CYP-PPAS is a safe vaccination adjuvant with high safety profile in vivo. Thus, the novel designed Pickering emulsion CYP-PPAS was a safe and effective adjuvant for inducing the strong and long-term immune response.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dioscorea / Nanopartículas Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2022 Tipo de documento: Article País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dioscorea / Nanopartículas Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2022 Tipo de documento: Article País de publicação: Holanda