Metagenomics next-generation sequencing tests take the stage in the diagnosis of lower respiratory tract infections.

Diao, Zhenli; Han, Dongsheng; Zhang, Rui; Li, Jinming

Diao, Zhenli; Han, Dongsheng; Zhang, Rui; Li, Jinming.

Afiliação

Diao Z; National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/ National Center of Gerontology, PR China.
Han D; Peking University Fifth School of Clinical Medicine, Beijing Hospital, Beijing, PR China.
Zhang R; Beijing Engineering Research Center of Laboratory Medicine, Beijing, PR China.
Li J; Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Clinical Laboratory, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China.

J Adv Res ; 38: 201-212, 2022 05.

Article em En | MEDLINE | ID: mdl-35572406

Assuntos

Microbiota; Infecções Respiratórias; Sequenciamento de Nucleotídeos em Larga Escala; Humanos; Metagenoma; Metagenômica; Microbiota/genética; Infecções Respiratórias/diagnóstico

Palavras-chave

ARGs, antibiotic resistance genes; CAP, Community-acquired pneumonia; CSF, Cerebrospinal fluid; DASH, Depletion of Abundant Sequences by Hybridization; DNBs, DNA nanoballs; DNase, Deoxyribonuclease; Fil, 5-µM filtration; IQC, Internal quality control; IQR, Interquartile range; LDTs, Laboratory-developed tests; LRIs, Lower respiratory tract infections; MTB, M. tuberculosis; Metagenomics; Mol, MolYsis™ Basic; NCBI, National Center for Biotechnology Information; NEB, NEBNext® Microbiome DNA Enrichment Kit; NPA, nasopharyngeal aspirate; Next generation sequencing; PMA, Propidium monoazide; PT, Proficiency testing; Pneumonia; QIA, QIAamp DNA Microbiome Kit; RMB, renminbi; RT-PCR, Reverse-transcription PCR; RVP, respiratory virus panel; Respiratory; RoC, Receiver-operating curve; SMRT, single-molecule real-time sequencing; TATs, Typical turnaround times; WGS, Whole-genome sequencing; mNGS; mNGS, Metagenomic next-generation sequencing

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Microbiota Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: J Adv Res Ano de publicação: 2022 Tipo de documento: Article País de publicação: Egito

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google