Your browser doesn't support javascript.
loading
Extraction, characterization, and biological activities of exopolysaccharides from plant root soil fungus Fusarium merismoides A6.
Chen, Guoqiang; Xu, Zhenxiang; Wang, Fan; Liu, Lijuan; Wei, Yuxi; Li, Jinglong; Zhang, Liang; Zheng, Kai; Wu, Lijun; Men, Xiao; Zhang, Haibo.
Afiliação
  • Chen G; CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
  • Xu Z; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Wang F; CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
  • Liu L; Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.
  • Wei Y; CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
  • Li J; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Zhang L; CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
  • Zheng K; College of Life Science, Qingdao University, Qingdao, 266071, China.
  • Wu L; Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.
  • Men X; Great Wall Cigar Factory Technology Development Center of China Tobacco Sichuan, Shifang, 618400, China.
  • Zhang H; Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China. jnzhengkai@163.com.
Braz J Microbiol ; 54(1): 199-211, 2023 Mar.
Article em En | MEDLINE | ID: mdl-36370337
The exploration of polysaccharides from microorganisms is of great importance. In this study, a new type of exopolysaccharide excreted by Fusarium merismoides A6 (FM-EPS) was isolated, and the extraction conditions were optimized using a response surface methodology (RSM). The extraction temperature at 0 °C, a precipitation time of 7.83 h, and an ethanol precipitation concentration of 77.64% were predicted and proved to be the best extraction conditions with the maximum extraction yield of 0.74 g/mL. Then, two fractions of F. merismoides A6 exopolysaccharides (FM-EPS1 and FM-EPS2) were obtained through DEAE Sepharose fast flow column chromatography. As indicated by monosaccharide composition analysis, both fractions mainly consisted of mannose, glucose, galactose, and ribose, with an average molecular weight of 5.14 × 104 and 6.50 × 104 g/mol, respectively. FT-IR and NMR spectroscopy indicated the FM-EPSs had both α- and ß-glycosidic bonds. Moreover, the determination of antioxidant and antiproliferative activities in vitro proved that FM-EPSs had good antioxidant activities and antiproliferation activities. FM-EPS1 showed stronger antioxidant activities than FM-EPS2. FM-EPS2 showed antiproliferation activities on HeLa and HepG2 cells, while FM-EPS1 had no obvious antiproliferative activity. Therefore, FM-EPSs could be explored as potential antioxidant and anticancer agent applied in food, feed, nutraceutical, pharmaceutical, cosmetics, and chemical industries.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fusarium / Antioxidantes Tipo de estudo: Prognostic_studies Idioma: En Revista: Braz J Microbiol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fusarium / Antioxidantes Tipo de estudo: Prognostic_studies Idioma: En Revista: Braz J Microbiol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Brasil