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Establishment and Characterization of a New Cell Line from Enzootic Nasal Adenocarcinoma in Goats: ENA-1.
Li, Lingxu; Tan, Weiye; Wang, Zhen; Guo, Wenqing; Yang, Deji; Yao, Dawei.
Afiliação
  • Li L; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
  • Tan W; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
  • Wang Z; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
  • Guo W; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
  • Yang D; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
  • Yao D; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Vet Sci ; 11(6)2024 Jun 07.
Article em En | MEDLINE | ID: mdl-38922007
ABSTRACT
Enzootic nasal adenocarcinoma (ENA) is a contagious tumor disease of goats and sheep, which is caused by enzootic nasal tumor virus (ENTV). To better understand the pathogenesis of ENA, this study aimed to establish a goat ENA cell line (ENA-1). The cells have been characterized with regard to morphology, growth rate, ultrastructural features, chromosome number, expression of CK7 and CK18, tumorigenicity, species, and mycoplasma contamination. ENA-1 had an epithelioid cell morphology with an unstable chromosome number under a light microscope. Under an electron microscope, the cell nuclear heterogeneity was not obvious, and there were more intermediate filaments and a small number of immature retrovirus-like particles in the cytoplasm. ENA-1 had strong proliferative potential, and the cell multiplication time was about 36 h, which could make BALB/c nude mice develop tumors. CK7 and CK18 were expressed in the cytoplasm of primary goat tumors, in transplanted tumors from nude mice, and un ENA-1 cells with the same intensity. PCR revealed that ENA-1 continuously carried ENTV-2 up to the 17th generation with no germline contamination or mycoplasma contamination. In conclusion, using a serum-containing culture system, ENA-1 cells were successfully isolated, cultured, and purified from goat tumor tissues. The isolated ENA-1 cells retained robust proliferation potential and maintained their phenotype, indicating the potential application of the ENA-1 cell line as an in vitro model of ENA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Vet Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Vet Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Suíça