Simultaneous detection of five mycotoxins in traditional Chinese medicines (TCMs) by visual protein microarray.
J Pharm Biomed Anal
; 249: 116333, 2024 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-39002187
ABSTRACT
The pollution of mycotoxins to crops such as traditional Chinese medicines (TCMs) is an established problem throughout the world. Thus, mycotoxin determination in TCMs during production and processing is significantly necessary, which means rapid, sensitive and accurate analytical methods are needed. In this work, a new method of visual protein microarray based on a 96-well microtiter plate was proposed. Combined with a colorimetric method, five mycotoxins (ochratoxin A, zearalenone, deoxynivalenol, aflatoxin B1 and fumonisin B1) in 90 samples (TCMs) could be detected simultaneously within 30â¯minutes. The detection limits for the five mycotoxins are 0.25⯵g/kg, 0.33⯵g/kg, 11.84⯵g/kg, 0.06⯵g/kg, and 3.58⯵g/kg, which can satisfy specified requirements of mycotoxins in the Chinese Pharmacopoeia (2020 edition) adequately. Under repeated conditions, experiments were carried out on actual samples to verify the feasibility of the method. The results showed that the recoveries of all analytes were between 70â¯% and 120â¯%, and the relative standard deviations were less than 15â¯%. In comparison to LC-MS/MS, this method significantly reduces the required time, and the colorimetric technique offers more direct results compared to fluorescence-based screening assays. This method exhibits substantial potential for the rapid and sensitive on-site detection of TCMs for quality control.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
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Contaminação de Medicamentos
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Colorimetria
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Análise Serial de Proteínas
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Limite de Detecção
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Micotoxinas
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Reino Unido