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Generation of B7-H3 isoform regulated by ANXA2/NSUN2/YBX1 axis in human glioma.
Shen, Yifen; Ma, Chunfang; Li, Xiangxiang; Li, Xiaosong; Wu, Yuxiang; Yang, Tao; Hu, Yanping; Liu, Chao; Shen, Hao; Guo, Pin; Shen, Yihang.
Afiliação
  • Shen Y; Central Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
  • Ma C; Clinical Laboratory, Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
  • Li X; Clinical Laboratory, Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
  • Li X; Department of Anorectal Surgery, Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
  • Wu Y; Department of Pathology, Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
  • Yang T; Department of Medical Cosmetology, Suzhou Ninth People's Hospital, Soochow University, Suzhou, Jiangsu, China.
  • Hu Y; Department of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, China.
  • Liu C; Central Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
  • Shen H; Clinical Laboratory, Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
  • Guo P; Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
  • Shen Y; Central Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
J Cell Mol Med ; 28(14): e18575, 2024 Jul.
Article em En | MEDLINE | ID: mdl-39048916
ABSTRACT
In recent years, in the development of emerging immunotherapy, B7-H3 is also termed as CD276 and has become a novel chimeric antigen receptor (CAR)-T target against glioma and other tumours, and aroused extensive attention. However, B7-H3 has three isoforms (2, 3 and 4Ig) with the controversial expression and elusive function in tumour especially glioma. The current study mainly focuses on the regulatory factors and related mechanisms of generation of different B7-H3 isoforms. First, we have determined that 2Ig is dominant in glioma with high malignancy, and 4Ig is widely expressed, whereas 3Ig shows negative expression in all glioma. Next, we have further found that RNA binding protein annexin A2 (ANXA2) is essential for B7-H3 isoform maintenance, but fail to determine the choice of 4Ig or 2Ig. RNA methyltransferase NOP2/Sun RNA methyltransferase 2 (NSUN2) and 5-methylcytosine reader Y-box binding protein 1 (YBX1) facilitate the production of 2Ig. Our findings have uncovered a series of factors (ANXA2/NSUN2/YBX1) that can determine the alternative generation of different isoforms of B7-H3 in glioma. Our result aims to help peers gain a clearer understanding of the expression and regulatory mechanisms of B7H3 in tumour patients, and to provide better strategies for designing B7H3 as a target in immunotherapy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação Neoplásica da Expressão Gênica / Anexina A2 / Isoformas de Proteínas / Antígenos B7 / Glioma Limite: Humans Idioma: En Revista: J Cell Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação Neoplásica da Expressão Gênica / Anexina A2 / Isoformas de Proteínas / Antígenos B7 / Glioma Limite: Humans Idioma: En Revista: J Cell Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido