Determining genetic diversity of prevalent G and P genotype of Bovine Rotavirus A from neonatal calves of Gujarat, India.
J Vet Sci
; 25(4): e55, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-39083207
ABSTRACT
IMPORTANCE Neonatal calf diarrhea is a major cause of mortality in newborn calves worldwide, posing a significant challenge in bovine herds. Group A Bovine Rotaviruses (BRVA) are the primary contributors to severe gastroenteritis in calves under two months old. OBJECTIVES:
This study examined the prevalence and molecular characterization of BRVA in neonatal calves in Gujarat, India.METHODS:
Sixty-nine diarrheic fecal samples were collected and subjected to various molecular methods of BRVA detection, isolation, and characterization.RESULTS:
The latex agglutination test (LAT), electropherotyping (RNA-PAGE), and reverse transcription polymerase chain reaction revealed positivity rates of 39.13%, 20.30%, and 37.70%, respectively. RNA-PAGE identified 11 bands with a 4232 migration pattern, indicative of the segmented genome of BRVA. BRVA was successfully isolated from LAT-positive samples, with 26 samples exhibiting clear cytopathic effects upon passage in MA-104 cell lines. Genotyping identified G10 as the predominant G genotype, with P[11] genotypes comprising 76.92% of the isolates. The most common G/P combination was G10P[11], highlighting its zoonotic potential. CONCLUSIONS AND RELEVANCE These findings underscore the importance of molecular detection and genotyping for effective vaccine development. This study provides crucial insights into the prevalent G and P genotypes of BRVA in Gujarat, India, aiding in the development of targeted control measures.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Rotavirus
/
Variação Genética
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Doenças dos Bovinos
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Rotavirus
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Genótipo
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Animais Recém-Nascidos
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Vet Sci
/
J. vet. sci
/
Journal of veterinary science
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Índia
País de publicação: