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Involvement of lipid-translocating exporter family proteins in determination of myriocin sensitivity in budding yeast.
Kawaguchi, Takahiro; Ishibashi, Yohei; Matsuzaki, Momoko; Yamagata, Satomi; Tani, Motohiro.
Afiliação
  • Kawaguchi T; Department of Chemistry, Faculty of Sciences, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
  • Ishibashi Y; Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
  • Matsuzaki M; Department of Chemistry, Faculty of Sciences, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
  • Yamagata S; Department of Chemistry, Faculty of Sciences, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
  • Tani M; Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Biochem Biophys Rep ; 39: 101785, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39104838
ABSTRACT
Myriocin is an inhibitor of serine palmitoyltransferase involved in the initial biosynthetic step for sphingolipids, and causes potent growth inhibition in eukaryotic cells. In budding yeast, Rsb1, Rta1, Pug1, and Ylr046c are known as the Lipid-Translocating Exporter (LTE) family and believed to contribute to export of various cytotoxic lipophilic compounds. It was reported that Rsb1 is a transporter responsible for export of intracellularly accumulated long-chain bases, which alleviate the cytotoxicity. In this study, it was found that LTE family genes are involved in determination of myriocin sensitivity in yeast. Analyses of effects of deletion and overexpression of LTE family genes suggested that all LTEs contribute to suppression of cytotoxicity of myriocin. It was confirmed that RSB1 overexpression suppressed reduction in complex sphingolipid levels caused by myriocin treatment, possibly exporting myriocin to outside of the cell. These results suggested that LTE family genes function as a defense mechanism against myriocin.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biochem Biophys Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biochem Biophys Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão País de publicação: Holanda