Exploring the resilience and stability of a defined human gut microbiota consortium: An isothermal microcalorimetric study.
Microbiologyopen
; 13(4): e1430, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-39115291
ABSTRACT
The gut microbiota significantly contributes to human health and well-being. The aim of this study was to evaluate the stability and resilience of a consortium composed of three next-generation probiotics (NGPs) candidates originally found in the human gut. The growth patterns of Akkermansia muciniphila, Bacteroides thetaiotaomicron, and Faecalibacterium prausnitzii were studied both individually and consortium. The growth kinetics of Akkermansia muciniphila (A. muciniphila), Bacteroides thetaiotaomicron (B. thetaiotaomicron), and Faecalibacterium prausnitzii (F. prausnitzii) were characterized both individually and in consortium using isothermal microcalorimetry and 16S ribosomal RNA next-generation sequencing. The consortium reached stability after three passages and demonstrated resilience to changes in its initial composition. The concentration of butyrate produced was nearly twice as high in the consortium compared to the monoculture of F. prausnitzii. The experimental conditions and methodologies used in this article are a solid foundation for developing further complex consortia.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Calorimetria
/
RNA Ribossômico 16S
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Microbioma Gastrointestinal
Limite:
Humans
Idioma:
En
Revista:
Microbiologyopen
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estônia
País de publicação:
Reino Unido