Your browser doesn't support javascript.
loading
Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18).
Schlichter Kadosh, Yael; Muthuraman, Subramani; Nisaa, Khairun; Ben-Zvi, Anat; Karsagi Byron, Danit Lisa; Shagan, Marilou; Brandis, Alexander; Mehlman, Tevie; Gopas, Jacob; Saravana Kumar, Rajendran; Kushmaro, Ariel.
Afiliação
  • Schlichter Kadosh Y; Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel.
  • Muthuraman S; Chemistry Division, Vellore Institute of Technology, Chennai, India.
  • Nisaa K; Department of Life Science, Ben-Gurion University of the Negev, Beer Sheva, Israel.
  • Ben-Zvi A; Department of Life Science, Ben-Gurion University of the Negev, Beer Sheva, Israel.
  • Karsagi Byron DL; Department of Civil and Environmental Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel.
  • Shagan M; Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel.
  • Brandis A; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
  • Mehlman T; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
  • Gopas J; Department of Microbiology, Immunology and Genetics Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.
  • Saravana Kumar R; Chemistry Division, Vellore Institute of Technology, Chennai, India.
  • Kushmaro A; Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel.
Biofilm ; 8: 100215, 2024 Dec.
Article em En | MEDLINE | ID: mdl-39148892
ABSTRACT
Bacterial communication, Quorum Sensing (QS), is a target against virulence and prevention of antibiotic-resistant infections. 16 derivatives of Piperlongumine (PL), an amide alkaloid from Piper longum L., were screened for QS inhibition. PL-18 had the best QSI activity. PL-18 inhibited the lasR-lasI, rhlR-rhlI, and pqs QS systems of Pseudomonas aeruginosa. PL-18 inhibited pyocyanin and rhamnolipids that are QS-controlled virulence elements. Iron is an essential element for pathogenicity, biofilm formation and resilience in harsh environments, its uptake was inhibited by PL-18. Pl-18 significantly reduced the biofilm biovolume including in established biofilms. PL-18-coated silicon tubes significantly inhibited biofilm formation. The transcriptome study of treated P. aeruginosa showed that PL-18 indeed reduced the expression of QS and iron homeostasis related genes, and up regulated sulfur metabolism related genes. Altogether, PL-18 inhibits QS, virulence, iron uptake, and biofilm formation. Thus, PL-18 should be further developed against bacterial infection, antibiotic resistance, and biofilm formation.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biofilm Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Israel País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biofilm Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Israel País de publicação: Holanda