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A Homolog of the Histidine Kinase RetS Controls the Synthesis of Alginates, PHB, Alkylresorcinols, and Motility in Azotobacter vinelandii.
Rosales-Cruz, Araceli; Reyes-Nicolau, Jimena; Minto-González, Eduardo; Meneses-Carbajal, Alan; Mondragón-Albarrán, Claudia; López-Pliego, Liliana; Castañeda, Miguel.
Afiliação
  • Rosales-Cruz A; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, IC-11 Ciudad Universitaria Puebla, Apdo. Postal 1622, C. P. 72000, Puebla, Pue, Mexico.
  • Reyes-Nicolau J; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, IC-11 Ciudad Universitaria Puebla, Apdo. Postal 1622, C. P. 72000, Puebla, Pue, Mexico.
  • Minto-González E; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, IC-11 Ciudad Universitaria Puebla, Apdo. Postal 1622, C. P. 72000, Puebla, Pue, Mexico.
  • Meneses-Carbajal A; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, IC-11 Ciudad Universitaria Puebla, Apdo. Postal 1622, C. P. 72000, Puebla, Pue, Mexico.
  • Mondragón-Albarrán C; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, IC-11 Ciudad Universitaria Puebla, Apdo. Postal 1622, C. P. 72000, Puebla, Pue, Mexico.
  • López-Pliego L; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, IC-11 Ciudad Universitaria Puebla, Apdo. Postal 1622, C. P. 72000, Puebla, Pue, Mexico.
  • Castañeda M; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, IC-11 Ciudad Universitaria Puebla, Apdo. Postal 1622, C. P. 72000, Puebla, Pue, Mexico. miguel.castaneda@correo.buap.mx.
Curr Microbiol ; 81(10): 311, 2024 Aug 17.
Article em En | MEDLINE | ID: mdl-39153035
ABSTRACT
The two-component system GacS/A and the posttranscriptional control system Rsm constitute a genetic regulation pathway in Gammaproteobacteria; in some species of Pseudomonas, this pathway is part of a multikinase network (MKN) that regulates the activity of the Rsm system. In this network, the activity of GacS is controlled by other kinases. One of the most studied MKNs is the MKN-GacS of Pseudomonas aeruginosa, where GacS is controlled by the kinases RetS and LadS; RetS decreases the kinase activity of GacS, whereas LadS stimulates the activity of the central kinase GacS. Outside of the Pseudomonas genus, the network has been studied only in Azotobacter vinelandii. In this work, we report the study of the RetS kinase of A. vinelandii; as expected, the phenotypes affected in gacS mutants, such as production of alginates, polyhydroxybutyrate, and alkylresorcinols and swimming motility, were also affected in retS mutants. Interestingly, our data indicated that RetS in A. vinelandii acts as a positive regulator of GacA activity. Consistent with this finding, mutation in retS also negatively affected the expression of small regulatory RNAs belonging to the Rsm family. We also confirmed the interaction of RetS with GacS, as well as with the phosphotransfer protein HptB.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Azotobacter vinelandii / Alginatos Idioma: En Revista: Curr Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Azotobacter vinelandii / Alginatos Idioma: En Revista: Curr Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos