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Dolosigranulum savutiense sp. nov., isolated from human upper respiratory samples collected in Botswana.
Popowitch, Elena B; Boiditswe, Sefelani C; Patel, Mohamed Z; Aquino, Jhoanna N; Sozat, A Kuzey; Caiazzo, Adrian J; Maldonado-Barragán, Antonio; Hurst, Jillian H; Steenhoff, Andrew P; Kelly, Matthew S.
Afiliação
  • Popowitch EB; Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA.
  • Boiditswe SC; Botswana-University of Pennsylvania Partnership, Gaborone, Botswana.
  • Patel MZ; School of Medicine, Faculty of Health Sciences, University of Botswana, Gaborone, Botswana.
  • Aquino JN; Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA.
  • Sozat AK; Duke University, Durham, North Carolina, USA.
  • Caiazzo AJ; Duke University, Durham, North Carolina, USA.
  • Maldonado-Barragán A; Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA.
  • Hurst JH; Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA.
  • Steenhoff AP; Botswana-University of Pennsylvania Partnership, Gaborone, Botswana.
  • Kelly MS; School of Medicine, Faculty of Health Sciences, University of Botswana, Gaborone, Botswana.
Article em En | MEDLINE | ID: mdl-39166976
ABSTRACT
Four strains (MSK211, MSK294T, MSK312, MSK433) of a novel Dolosigranulum species were cultured from nasopharyngeal swabs collected from mother-infant dyads in southern Botswana. These strains grew optimally on tryptic soy agar with 5% sheep blood solid medium and in fastidious bacteria broth. Colonies on tryptic soy agar with 5% sheep blood agar appeared grey or white with a flat, smooth surface and variable alpha haemolysis. Cells were Gram-positive, non-spore-forming, non-motile cocci that lacked catalase or oxidase activity. Major fatty acids were C16  0 (palmitic acid), C18  1 ω9c (oleic acid), and C18  0 (stearic acid). Analyses of 16S rRNA gene sequences identified these strains as belonging to the genus Dolosigranulum (family Carnobacteriaceae), which currently contains only a single validly published species (Dolosigranulum pigrum). Whole-genome sequencing revealed that the genomes of these strains are 1.98-2.07 Mbp in size and have a G+C content of 39.6-39.9 mol%. Comparisons of these genomes to publicly available genomes of D. pigrum yielded average nucleotide identities and in silico DNA-DNA hybridization values of 92.3-92.9% and 49.1-51.4%, respectively. These results indicate that these strains represent a novel species of Dolosigranulum, for which we propose the name Dolosigranulum savutiense sp. nov., with the type strain MSK294T (=DSM 117171T=JCM 36673T).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Composição de Bases / DNA Bacteriano / RNA Ribossômico 16S / Técnicas de Tipagem Bacteriana / Análise de Sequência de DNA / Ácidos Graxos / Hibridização de Ácido Nucleico Limite: Female / Humans / Infant País/Região como assunto: Africa Idioma: En Revista: Int J Syst Evol Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Composição de Bases / DNA Bacteriano / RNA Ribossômico 16S / Técnicas de Tipagem Bacteriana / Análise de Sequência de DNA / Ácidos Graxos / Hibridização de Ácido Nucleico Limite: Female / Humans / Infant País/Região como assunto: Africa Idioma: En Revista: Int J Syst Evol Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido