Antibacterial activity of a polysaccharide isolated from litchi (Litchi chinensis Sonn.) pericarp against Staphylococcus aureus and the mechanism investigation.
Int J Biol Macromol
; 279(Pt 1): 134788, 2024 Nov.
Article
em En
| MEDLINE
| ID: mdl-39173786
ABSTRACT
The long-term use of antibiotics can cause drug resistance. Natural polysaccharides are a novel means of treating bacterial infections, and the development and utilization of litchi pericarp polysaccharide (LPPs) as a bacteriostatic active substance offer a new research direction for the high-value utilization of litchi by-products. This study revealed that LPPs inhibited Staphylococcus aureus more than Escherichia coli, Listeria monocytogenes, and Salmonella typhimurium, with the minimum inhibitory concentrations of 145, 205, 325, and 445 µg/mL, respectively. The inhibitory activity of LPPs was insignificant for Bacillus subtilis at 505 µg/mL. The assessment of antibacterial mechanisms revealed that LPPs influenced the growth, conductivity, protein, and nucleic acid, reducing sugar, respiratory chain dehydrogenase activity, bacterial lipid peroxidation, intracellular adenosine triphosphate, and extracellular alkaline phosphatase levels of S. aureus. Of note, LPPs could modify the cell wall integrity and cell membrane permeability of S. aureus, resulting in the leakage of intracellular large and small molecules, inhibition of cellular respiratory metabolism, and oxidative losses. These processes exhibited an inhibitory effect and made the bacterium nonfunctional, thereby affecting its growth and metabolism or causing cell death. These findings provide support and insights into the potential application of LPPs as a natural antimicrobial agent.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Staphylococcus aureus
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Testes de Sensibilidade Microbiana
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Litchi
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Antibacterianos
Idioma:
En
Revista:
Int J Biol Macromol
/
Int. j. biol. macromol
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International journal of biological macromolecules
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
Holanda