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Effect of bovine beta-casomorphins on rat pancreatic beta cells (RIN-5F) under glucotoxic stress.
Dubey, Shivam Kumar; Thakur, Abhishek; Jena, Manoj Kumar; Kumar, Sudarshan; Sodhi, Monika; Mukesh, Manishi; Kaushik, Jai Kumar; Mohanty, Ashok Kumar.
Afiliação
  • Dubey SK; Cell, Molecular and Proteomics Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (ICAR-NDRI), Karnal, Haryana, 132001, India. Electronic address: shivamkumard84@gmail.com.
  • Thakur A; Cell, Molecular and Proteomics Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (ICAR-NDRI), Karnal, Haryana, 132001, India. Electronic address: thakurabhishek024@gmail.com.
  • Jena MK; Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 144411, India. Electronic address: manoj.20283@lpu.co.in.
  • Kumar S; Cell, Molecular and Proteomics Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (ICAR-NDRI), Karnal, Haryana, 132001, India. Electronic address: kumarsudershan@gmail.com.
  • Sodhi M; Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources (ICAR-NBAGR), Karnal, Haryana, 132001, India. Electronic address: monikasodhi@yahoo.com.
  • Mukesh M; Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources (ICAR-NBAGR), Karnal, Haryana, 132001, India. Electronic address: mmukesh_26@hotmail.com.
  • Kaushik JK; Cell, Molecular and Proteomics Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (ICAR-NDRI), Karnal, Haryana, 132001, India. Electronic address: jaikr1@gmail.com.
  • Mohanty AK; Cell, Molecular and Proteomics Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (ICAR-NDRI), Karnal, Haryana, 132001, India; ICAR-Central Institute for Research on Cattle (ICAR-CIRC), Meerut, Uttar Pradesh, 250001, India. Electronic address: ashokmohanty1@gmail.com.
Biochem Biophys Res Commun ; 739: 150578, 2024 Aug 22.
Article em En | MEDLINE | ID: mdl-39178795
ABSTRACT
Beta-casomorphins (BCMs) are the bio-active peptides having opioid properties which are formed by the proteolytic digestion of ß-caseins in milk. BCM-7 forms when A1 milk is digested in the small intestine due to a histidine at the 67th position in ß-casein, unlike A2 milk, which has proline at this position and produces BCM-9. BCM-7 has further degraded into BCM-5 by the dipeptidyl peptidase-IV (DPP-IV) enzyme in the intestine. The opioid-like activity of BCM-7 is responsible for eliciting signaling pathways which enable a wide range of physiological effects. The aim of our study was to find out the differential role of BCMs (BCM-7, BCM-9 and BCM-5) on pancreatic ß-cell proliferation, insulin secretion, and opioid peptide binding receptors from ß-cells (RIN-5F cell line) in normal (5.5 mM) and high glucose (27.5 mM) concentrations. Our results showed that BCM-7/9/5 did not affect ß-cell viability, proliferation, and insulin secretion at normal glucose level. However, at higher glucose concentration, BCMs significantly protected ß-cells from glucotoxicity but did not affect the insulin secretion. Interestingly, in the presence of Mu-opioid peptide receptor antagonist CTOP, BCMs did not protect ß-cells from glucotoxicity. The results suggest that BCMs protect ß-cells from glucotoxicity via non-opioid mediated pathways because BCMs did not modulate the gene expression of the mu, kappa and delta opioid peptide receptors.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos