Optimization of the 5' untranslated region of mRNA vaccines.
Sci Rep
; 14(1): 19845, 2024 08 27.
Article
em En
| MEDLINE
| ID: mdl-39191885
ABSTRACT
To investigate the impact of different 5' untranslated regions (UTRs) on mRNA vaccine translation efficiency, five dual-reporter gene expression plasmids with different 5'UTRs were constructed. The corresponding mRNA transcripts were transcribed and capped in vitro. By comparing the expression levels of reporter genes with different 5'UTRs, we identified the 5'UTR associated with the highest expression level. Subsequently, HIVgp145 mRNA vaccines containing various 5'UTRs were constructed and verified. The results demonstrated that mRNA 3 (ß-globin 5'UTR) displayed the greatest number of green fluorescence-positive cells and the highest luciferase fluorescence intensity in the reporter gene expression system. Further, among the HIVgp145 mRNA vaccines with different 5'UTRs, mRNA 7 (ß-globin 5'UTR) exhibited the highest level of expression. These findings indicate that it is feasible to use the 5'UTR of ß-globin in an mRNA vaccine, laying the foundation for animal immunogenicity testing.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes Reporter
/
Regiões 5' não Traduzidas
/
Vacinas de mRNA
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China