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Acute waterborne cadmium exposure induces liver ferroptosis in Channa argus.
Chen, Xingyu; Sun, Wenqian; Song, Yanting; Wu, Shangong; Xie, Shouqi; Xiong, Wen; Peng, Chengdong; Peng, Yu; Wang, Zhengxiang; Lek, Sovan; Hogstrand, Christer; Sørensen, Mette; Pan, Lei; Liu, Dong.
Afiliação
  • Chen X; Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China; Hubei Key Laboratory of Regional Development and Environmental Response, Hubei University, Wuhan 430062, China.
  • Sun W; Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China; Hubei Key Laboratory of Regional Development and Environmental Response, Hubei University, Wuhan 430062, China.
  • Song Y; Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China; Hubei Key Laboratory of Regional Development and Environmental Response, Hubei University, Wuhan 430062, China.
  • Wu S; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China.
  • Xie S; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China.
  • Xiong W; College of Life Sciences, Hubei Normal University, Huangshi 435002, China.
  • Peng C; Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
  • Peng Y; Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China; Hubei Key Laboratory of Regional Development and Environmental Response, Hubei University, Wuhan 430062, China.
  • Wang Z; Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China; Hubei Key Laboratory of Regional Development and Environmental Response, Hubei University, Wuhan 430062, China.
  • Lek S; Laboratoire Evolution & Diversité Biologique, UMR 5174, Université Paul Sabatier-Toulouse III, 118 routes de Narbonne, 31062, Toulouse Cedex 4, France.
  • Hogstrand C; Department of Nutritional Sciences, King's College London, London WC2R 2LS, UK.
  • Sørensen M; Faculty of Biosciences and Aquaculture, Nord University, Bodø 8049, Norway.
  • Pan L; Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China; Hubei Key Laboratory of Regional Development and Environmental Response, Hubei University, Wuhan 430062, China. Electronic address: leipan@hubu.edu.cn.
  • Liu D; Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: liudong0826@hust.edu.cn.
Ecotoxicol Environ Saf ; 283: 116947, 2024 Sep 15.
Article em En | MEDLINE | ID: mdl-39213749
ABSTRACT
The impact of cadmium (Cd) toxicity on fish liver injury has received much attention in recent years. Currently, autophagy, apoptosis and endoplasmic reticulum stress were reported in Cd exposed fish liver, and if there are other mechanisms (such as ferroptosis) and relevant signaling pathways involved in fish remains unknown. An experiment was conducted to investigate Cd toxicity in Channa argus (Cantor, 1842) exposed to 0, 1.0, and 2.0 mg Cd/L of water for 96 h. Cd disrupted the structure of mitochondria in the liver. Besides, Cd induced ferroptosis by significantly increasing the level of Fe2+, ROS, MDA and significantly decreasing the level of Ferritin, GSH, GSH-Px, GPX4, GST and SOD (p < 0.05 in all cases). In addition, the mRNA expression of ferroptosis related genes, gpx4 and slc7a11, were significantly downregulated by Cd. Moreover, Cd exposure significantly inhibited the Nrf2/Keap1 signaling pathway, one of the pathways involved in ferroptosis, by upregulating the mRNA levels of keap1a and keap1b, and downregulating the mRNA levels of nrf2 and its target genes (ho-1, nqo1 and cat). Cd exposure also caused extensive accumulation of vacuoles and lipid droplets in liver, as well as an increase in triglyceride content. Cd significantly affected lipid metabolism related enzyme activity and gene expression, which were also regulated by Nrf2/Keap1 signaling pathway. In summary, these results indicate that ferroptosis is a mechanism in waterborne Cd exposed fish liver injury via the Nrf2/Keap1 signaling pathway and the Cd induced hepatic steatosis is also modulated by Nrf2/Keap1 pathway at the whole-body level in fish. These findings provide new insights into the fish liver injury and molecular basis of Cd toxicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Cádmio / Peixes / Ferroptose / Fígado Limite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2024 Tipo de documento: Article País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Cádmio / Peixes / Ferroptose / Fígado Limite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2024 Tipo de documento: Article País de publicação: Holanda