Identifying Putative Biomarkers of Foodborne Pathogens Using a Metabolomic Approach.
Methods Mol Biol
; 2852: 255-272, 2025.
Article
em En
| MEDLINE
| ID: mdl-39235749
ABSTRACT
Metabolomics is the study of low molecular weight biochemical molecules (typically <1500 Da) in a defined biological organism or system. In case of food systems, the term "food metabolomics" is often used. Food metabolomics has been widely explored and applied in various fields including food analysis, food intake, food traceability, and food safety. Food safety applications focusing on the identification of pathogen-specific biomarkers have been promising. This chapter describes a nontargeted metabolite profiling workflow using gas chromatography coupled with mass spectrometry (GC-MS) for characterizing three globally important foodborne pathogens, Escherichia coli O157H7, Listeria monocytogenes, and Salmonella enterica, from selective enrichment liquid culture media. The workflow involves a detailed description of food spiking experiments followed by procedures for the extraction of polar metabolites from media, the analysis of the extracts using GC-MS, and finally chemometric data analysis using univariate and multivariate statistical tools to identify potential pathogen-specific biomarkers.
Palavras-chave
Food safety; Foodborne pathogens; Gas chromatography-mass spectrometry (GC-MS); MetaboAnalyst; Metabolomic profiling; Partial least square-discriminant analysis (PLS-DA); Principal component analysis (PCA); Receiver operating characteristic area under the curve (ROC-AUC) analysis; SIMCA; Significant analysis of metabolomics (SAM) plots
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biomarcadores
/
Metabolômica
/
Microbiologia de Alimentos
/
Listeria monocytogenes
/
Cromatografia Gasosa-Espectrometria de Massas
Idioma:
En
Revista:
Methods Mol Biol
/
Methods in molecular biology
/
Methods mol. biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2025
Tipo de documento:
Article
País de afiliação:
Austrália
País de publicação:
Estados Unidos