Your browser doesn't support javascript.
loading
Characterization of age-associated inflammasome activation reveals tissue specific differences in transcriptional and post-translational inflammatory responses.
Talley, Sarah; Nguyen, Tyler; Van Ye, Lily; Valiauga, Rasa; DeCarlo, Jake; Mustafa, Jabra; Cook, Benjamin; White, Fletcher A; Campbell, Edward M.
Afiliação
  • Talley S; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA.
  • Nguyen T; Department of Anesthesia, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Van Ye L; Richard L. Roudebush VA Medical Center, Indianapolis, IN, USA.
  • Valiauga R; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA.
  • DeCarlo J; Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USA.
  • Mustafa J; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA.
  • Cook B; Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USA.
  • White FA; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA.
  • Campbell EM; Department of Anesthesia, Indiana University School of Medicine, Indianapolis, IN, USA.
Immun Ageing ; 21(1): 60, 2024 Sep 10.
Article em En | MEDLINE | ID: mdl-39256821
ABSTRACT
Aging is associated with systemic chronic, low-grade inflammation, termed 'inflammaging'. This pattern of inflammation is multifactorial and is driven by numerous inflammatory pathways, including the inflammasome. However, most studies to date have examined changes in the transcriptomes that are associated with aging and inflammaging, despite the fact that inflammasome activation is driven by a series of post-translational activation steps, culminating in the cleavage and activation of caspase-1. Here, we utilized transgenic mice expressing a caspase-1 biosensor to examine age-associated inflammasome activation in various organs and tissues to define these post-translational manifestations of inflammaging. Consistent with other studies, we observe increased inflammation, including inflammasome activation, in aged mice and specific tissues. However, we note that the degree of inflammasome activation is not uniformly associated with transcriptional changes commonly used as a surrogate for inflammasome activation in tissues. Furthermore, we used a skull thinning technique to monitor central nervous system inflammasome activation in vivo in aged mice and found that neuroinflammation is significantly amplified in aged mice in response to endotoxin challenge. Together, these data reveal that inflammaging is associated with both transcriptional and post-translational inflammatory pathways that are not uniform between tissues and establish new methodologies for measuring age-associated inflammasome activation in vivo and ex vivo.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Immun Ageing Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Immun Ageing Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido