Your browser doesn't support javascript.
loading
Incorporation of nitrogen in antinutritional Solanum alkaloid biosynthesis.
Grzech, Dagny; Smit, Samuel J; Alam, Ryan M; Boccia, Marianna; Nakamura, Yoko; Hong, Benke; Barbole, Ranjit; Heinicke, Sarah; Kunert, Maritta; Seibt, Wibke; Grabe, Veit; Caputi, Lorenzo; Lichman, Benjamin R; O'Connor, Sarah E; Aharoni, Asaph; Sonawane, Prashant D.
Afiliação
  • Grzech D; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Smit SJ; Centre for Novel Agricultural Products, Department of Biology, University of York, York, UK.
  • Alam RM; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Boccia M; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Nakamura Y; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Hong B; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Barbole R; Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.
  • Heinicke S; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Kunert M; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Seibt W; Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Grabe V; Microscopic Imaging Service Group, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Caputi L; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
  • Lichman BR; Centre for Novel Agricultural Products, Department of Biology, University of York, York, UK.
  • O'Connor SE; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany. oconnor@ice.mpg.de.
  • Aharoni A; Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel. asaph.aharoni@weizmann.ac.il.
  • Sonawane PD; Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany. psonawane@ice.mpg.de.
Nat Chem Biol ; 2024 Sep 13.
Article em En | MEDLINE | ID: mdl-39271954
ABSTRACT
Steroidal glycoalkaloids (SGAs) are specialized metabolites produced by hundreds of Solanum species including food crops, such as tomato, potato and eggplant. Unlike true alkaloids, nitrogen is introduced at a late stage of SGA biosynthesis through an unknown transamination reaction. Here, we reveal the mechanism by which GLYCOALKALOID METABOLISM12 (GAME12) directs the biosynthesis of nitrogen-containing steroidal alkaloid aglycone in Solanum. We report that GAME12, a neofunctionalized γ-aminobutyric acid (GABA) transaminase, undergoes changes in both active site specificity and subcellular localization to switch from its renown and generic activity in core metabolism to function in a specialized metabolic pathway. Moreover, overexpression of GAME12 alone in engineered S. nigrum leaves is sufficient for de novo production of nitrogen-containing SGAs. Our results highlight how hijacking a core metabolism GABA shunt enzyme is crucial in numerous Solanum species for incorporating a nitrogen to a steroidal-specialized metabolite backbone and form defensive alkaloids.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nat Chem Biol Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nat Chem Biol Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Estados Unidos