Phenotypic Changes in Phage Survivors of Multidrug-Resistant Klebsiella pneumoniae.
Indian J Microbiol
; 64(3): 1379-1383, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-39282179
ABSTRACT
Multidrug-resistant Klebsiella pneumoniae (MDR-KP) infections have become a major global issue in the healthcare sector. Alternative viable tactics for combating bacterial infections, such as the use of bacteriophages, can be considered. One of the major challenges in phage therapy is the emergence of phage-resistant bacteria. This study isolated bacteriophages from water and soil samples against MDR-KP isolates. Susceptible bacterial hosts were exposed to phages at different concentrations and prolonged durations of time to obtain phage-resistant survivors. Phenotypic changes such as changes in growth rates, biofilm formation ability, antibiotic sensitivity patterns, and outer membrane proteins (OMPs) profiling of the survivors were studied. Our findings indicate that the phage ØKp11 and ØKp26 survivors had reduced growth rates and biofilm formation ability, altered antibiotic sensitivity patterns, and reduced OMPs expression compared with the parent MDR-KP002 isolate. These results suggest that the alternations in the bacterial envelope result in phenotypic phage resistance among MDR bacterial isolates. Supplementary Information The online version contains supplementary material available at 10.1007/s12088-024-01217-6.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Indian J Microbiol
/
Indian journal of microbiology
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Índia