Removal of polyphenols from anthocyanin-rich extracts using 4-vinylpyridine crosslinked copolymers.
Food Chem
; 463(Pt 2): 141312, 2024 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-39305668
ABSTRACT
In this work, new sorbents for the purification of anthocyanin-rich extracts were evaluated. Copolymers of 4-vinylpyridine crosslinked with trimethylolpropane trimethacrylate (poly(4VP-co-TRIM)) or 1,4-dimethacryloyloxybenzene (poly(4VP-co-14DMB)) were tested for their potential to capture polyphenols. Copolymers were obtained by seed swelling polymerization in the form of microspheres with permanent porous structure - attractive features of sorbents used for sample purification by dispersive solid phase extraction. The microspheres were characterized by AFM, elemental analysis, SEM, and nitrogen adsorption-desorption method. Their capacity to remove polyphenols was evaluated using spectrophotometry, HPLC-DAD, and LC-MS/MS. For proof-of-concept, the aqueous extracts of berries classified into three different groups regarding their anthocyanin composition (strawberries, raspberries, blackcurrants) were selected. It was found that studied microspheres adsorbed flavonoids more effectively compared to primary secondary amine and graphitized carbon black. Copolymers of 4-vinylpyridine also capture anthocyanins and might be used for the purification of extracts of fruits before LC-MS/MS analysis to reduce the matrix effect.
4-hydroxybenzoic acid (PubChem CID: 135); Anthocyanins; Berry fruit; Caffeic acid (PubChem CID: 689043); Catechin (PubChem CID: 73160); Chlorogenic acid (PubChem CID: 1794427); Dispersive solid phase extraction; Ellagic acid (PubChem CID: 5281855); Ferulic acid (PubChem CID: 445858); Flavonoids; Gallic acid (PubChem CID: 370); Myricetin (PubChem CID: 5281672); Polymeric materials; Quercetin (PubChem CID: 5280343); Rutin (PubChem CID: 5280805)
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Reino Unido