Based on Serum Pharmacochemistry and Metabolomics Studied the Pharmacodynamic Material Basis and Mechanism of Rubi Fructus (Fupenzi) in Improving the Symptom of Kidney-Yang Deficiency.

ABSTRACT

Rubi fructus (Fupenzi) is the Chinese medicine for both food and medicine, which can be used to tonify kidney yang, strengthen essence and shrink urine, but its effective components and mechanism are not clear. In this paper, the active components of Fupenzi in vivo and in vitro were detected. Adenine was used to replicate the model of kidney yang deficiency, and organ index, biochemical index and histopathology were used to evaluate the effect of different doses of Fupenzi on tonifying kidney yang. Metabonomics technique was used to analyze the metabolic regulation mechanism of Fupenzi in improving kidney yang deficiency syndrome. The results showed that 61 chemical constituents of Fupenzi were identified in vitro. A total of 51 chemical components were identified, including 30 prototype components and 21 metabolic components, which may be theeffective components of Fupenzi. The results of pharmacodynamics showed that Fupenzi can effectively improve the symptom of kidney-yang deficiency, which may be mainly through primary bile acid biosynthesis, linoleic acid metabolism, steroid hormone biosynthesis, ß-alanine metabolism, glutathione metabolism, porphyrin and chlorophyll metabolism, unsaturated fatty acid biosynthesis, arachidonic acid metabolism, arginine and proline metabolism and other metabolic pathways to improve adenine-induced metabolic disorders in rats with kidney-yang deficiency syndrome.