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Detection and Molecular Characterization of GI-1 and GI-23 Avian Infectious Bronchitis Virus in Broilers Indicate the Emergence of New Genotypes in Bolivia.
Villanueva-Pérez, Doris; Tataje-Lavanda, Luis; Montalván-Avalos, Angela; Paredes-Inofuente, Diego; Montoya-Ortiz, Suly; Isasi-Rivas, Gisela; Fernández, María F; Fernández-Sánchez, Manolo; Fernández-Díaz, Manolo.
Afiliação
  • Villanueva-Pérez D; Research and Development Laboratories, FARVET, Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta 11702, Peru.
  • Tataje-Lavanda L; Research and Development Laboratories, FARVET, Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta 11702, Peru.
  • Montalván-Avalos A; Escuela Profesional de Medicina Humana, Universidad Privada San Juan Bautista, Lima 15067, Peru.
  • Paredes-Inofuente D; Research and Development Laboratories, FARVET, Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta 11702, Peru.
  • Montoya-Ortiz S; Research and Development Laboratories, FARVET, Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta 11702, Peru.
  • Isasi-Rivas G; Research and Development Laboratories, FARVET, Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta 11702, Peru.
  • Fernández MF; Research and Development Laboratories, FARVET, Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta 11702, Peru.
  • Fernández-Sánchez M; Research and Development Laboratories, FARVET, Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta 11702, Peru.
  • Fernández-Díaz M; Research and Development Laboratories, FARVET, Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta 11702, Peru.
Viruses ; 16(9)2024 Sep 14.
Article em En | MEDLINE | ID: mdl-39339939
ABSTRACT
Infectious Bronchitis Virus (IBV) is a major threat to the poultry industry worldwide, causing significant economic losses. While the virus's genetic structure is well understood, the specific strains circulating in Bolivia have remained uncharacterized until now. This study aimed to identify and characterize new IBV strains in Bolivia. Tissue samples from broilers exhibiting clinical signs of Infectious Bronchitis were screened to detect IBV using real-time RT-PCR (RT-qPCR). Positive samples with low cycle threshold (Ct) values were selected for sequencing the full S1 gene. Of the 12 samples analyzed, 10 were determined to be positive for IBV. However, only four samples yielded sufficient genetic material for sequencing and subsequent phylogenetic analysis. The results revealed the presence of GI-1 and GI-23 lineages, both belonging to genotype I (GI). The GI-1 lineage showed >99% sequence identity to the H120 and Massachusetts vaccine strains, suggesting a close relationship. In contrast, the GI-23 lineage clustered with other IBV strains, showing a distinct subclade that is genetically distant from Brazilian strains. No evidence of recombination was found. Furthermore, amino acid substitution analysis identified specific mutations in the S1 subunit, particularly in the hypervariable regions 1, 2, and 3. These mutations could potentially alter the virus's antigenicity, leading to reduced vaccine efficacy. The findings of this study highlight the importance of continued and broad genomic surveillance of circulating IBV strains and the need to improve vaccination strategies in Bolivia.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Doenças das Aves Domésticas / Galinhas / Infecções por Coronavirus / Vírus da Bronquite Infecciosa / Genótipo Limite: Animals País/Região como assunto: America do sul / Bolivia Idioma: En Revista: Viruses Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Peru País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Doenças das Aves Domésticas / Galinhas / Infecções por Coronavirus / Vírus da Bronquite Infecciosa / Genótipo Limite: Animals País/Região como assunto: America do sul / Bolivia Idioma: En Revista: Viruses Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Peru País de publicação: Suíça