AP endonuclease 1: Biological updates and advances in activity analysis.
Methods Enzymol
; 705: 347-376, 2024.
Article
em En
| MEDLINE
| ID: mdl-39389669
ABSTRACT
Apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1, APEX1, REF1, HAP1) is an abasic site-specific endonuclease holding critical roles in numerous biological functions including base excision repair, the DNA damage response, redox regulation of transcription factors, RNA processing, and gene regulation. Pathologically, APE1 expression and function is linked with numerous human diseases including cancer, highlighting the importance of sensitive and quantitative assays to measure APE1 activity. Here, we summarize biochemical and biological roles for APE1 and expand on the discovery of APE1 inhibitors. Finally, we highlight the development of assays to monitor APE1 activity, detailing a recently improved and stabilized DNA Repair Molecular Beacon assay to analyze APE1 activity. The assay is amenable to analysis of purified protein, to measure changes in APE1 activity in cell lysates, to monitor human patient samples for defects in APE1 function, or the cellular and biochemical response to APE1 inhibitors.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Liase (Sítios Apurínicos ou Apirimidínicos)
/
Reparo do DNA
/
Inibidores Enzimáticos
/
Ensaios Enzimáticos
Limite:
Humans
Idioma:
En
Revista:
Methods Enzymol
/
Methods enzymol
/
Methods in enzymology
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos
País de publicação:
Estados Unidos