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Round-cell mutants of Salmonella typhimurium produced by transposition mutagenesis: lethality of rodA and mre mutations.
Costa, C S; Antón, D N.
Afiliação
  • Costa CS; Departamento de Radiobiología, Comisión Nacional de Energía Atómica, Buenos Aires, Argentina.
Mol Gen Genet ; 236(2-3): 387-94, 1993 Jan.
Article em En | MEDLINE | ID: mdl-8382342
Thirty-three insertions of transposon Tn10 delta 16 delta 17 into genes involved in the control of rod cell shape were isolated in Salmonella typhimurium by the characteristic glossy appearance of colonies composed of spherical cells. Genetic tests demonstrated that 25 (76%) were insertions in the rodA gene, 7 (21%) were mre mutants, and 1 (3%) was a divD mutant. No insertion in the pbpA gene were found. Insertions in cell shape genes only appeared when strains displaying resistance to mecillinam (not caused by beta-lactamase production) were employed. Neither rodA nor mre insertions could be transduced to wild-type strains but they were normally accepted by mecillinam-resistant derivatives and by cya and crp mutants, which, unlike the corresponding Escherichia coli strains, did not display resistance to mecillinam. On the other hand, the divD insertion could be efficiently transduced to any strain. It is concluded that the rodA, mre, and divD genes are involved in the control of rod cell shape but, in addition, the RodA and Mre products perform some function(s) that is essential for wild-type cells but dispensable for some mecillinam-resistant strains, and for cya and crp mutants.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhimurium / Mutagênese Insercional / Proteínas de Escherichia coli / Genes Letais / Proteínas de Membrana / Morfogênese Idioma: En Revista: Mol Gen Genet Ano de publicação: 1993 Tipo de documento: Article País de afiliação: Argentina País de publicação: Alemanha
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhimurium / Mutagênese Insercional / Proteínas de Escherichia coli / Genes Letais / Proteínas de Membrana / Morfogênese Idioma: En Revista: Mol Gen Genet Ano de publicação: 1993 Tipo de documento: Article País de afiliação: Argentina País de publicação: Alemanha