Separation of toxoplasma cysts from brain tissue and liberation of viable bradyzoites.
J Parasitol
; 81(6): 1008-10, 1995 Dec.
Article
em En
| MEDLINE
| ID: mdl-8544039
A method was developed to separate Toxoplasma gondii cysts from mouse brain tissue and to liberate intact bradyzoites from cysts. Brains were blended in a Waring blender with 20% dextran solution and the homogenate was centrifuged at 4,000 g for 10 min. Cysts present in the sediment were digested by adding an equal amount of a solution containing 1 g NaCl, 1.4 ml HCl, and 1 mg pepsin (1:60,000 assay activity) per 100 ml water, and incubated at 37 C for 1 min. This method is harmless for bradyzoites, as tested by bioassays in mice. It compares favorably with published methods for separating cysts that require 3 centrifugations to achieve similar results.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxoplasma
/
Encéfalo
/
Separação Celular
/
Toxoplasmose Animal
Limite:
Animals
Idioma:
En
Revista:
J Parasitol
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Uruguai
País de publicação:
Estados Unidos