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Separation of toxoplasma cysts from brain tissue and liberation of viable bradyzoites.
Freyre, A.
Afiliação
  • Freyre A; Department of Parasitology, College for Veterinary Sciences, Montevideo, Uruguay.
J Parasitol ; 81(6): 1008-10, 1995 Dec.
Article em En | MEDLINE | ID: mdl-8544039
A method was developed to separate Toxoplasma gondii cysts from mouse brain tissue and to liberate intact bradyzoites from cysts. Brains were blended in a Waring blender with 20% dextran solution and the homogenate was centrifuged at 4,000 g for 10 min. Cysts present in the sediment were digested by adding an equal amount of a solution containing 1 g NaCl, 1.4 ml HCl, and 1 mg pepsin (1:60,000 assay activity) per 100 ml water, and incubated at 37 C for 1 min. This method is harmless for bradyzoites, as tested by bioassays in mice. It compares favorably with published methods for separating cysts that require 3 centrifugations to achieve similar results.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxoplasma / Encéfalo / Separação Celular / Toxoplasmose Animal Limite: Animals Idioma: En Revista: J Parasitol Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Uruguai País de publicação: Estados Unidos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxoplasma / Encéfalo / Separação Celular / Toxoplasmose Animal Limite: Animals Idioma: En Revista: J Parasitol Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Uruguai País de publicação: Estados Unidos