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Regulatory role of angiotensin II on progesterone production by cultured human granulosa cells. Expression of angiotensin II type-2 receptor.
Johnson, M C; Vega, M; Vantman, D; Troncoso, J L; Devoto, L.
Afiliação
  • Johnson MC; Institute of Maternal and Child Research, School of Medicine, University of Chile, San Borja-Arriarán Clinical Hospital, Santiago, Chile.
Mol Hum Reprod ; 3(8): 663-8, 1997 Aug.
Article em En | MEDLINE | ID: mdl-9294849
The role of angiotensin II (AngII) in ovarian steroidogenesis is not clearly understood. In order to study its action on progesterone synthesis and to determine which receptor subtype is involve, granulosa cells obtained from women undergoing in-vitro fertilization were cultured for 2 or 4 days and then incubated in the presence of AngII (10(-7) M) with or without human chorionic gonadotrophin (HCG, 10 IU/ml) for 3 or 18 h. In cells cultured for 2 days, incubation with AngII decreased progesterone secretion by 36%, and inhibited activity of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) by 87% (P < 0.05), although its expression was not significantly reduced. However, in cells cultured for 4 days, progesterone production was enhanced by incubation with AngII (38%), and no change was observed in 3 beta-HSD expression. Both inhibitory and stimulatory effects were dose-dependent. Progesterone secretion was increased (93%) by incubation with HCG of cells cultured for 4, but not for 2 days, and no potentiation was observed with AngII. Treatment with PD123177 completely blocked the action of AngII and decreased the HCG-stimulated secretion of progesterone by 27%. Angiotensin type-2 (AT2) receptor mRNA was expressed in cells cultured for 4 days. In conclusion, AngII showed a regulatory role in in-vitro progesterone production by human granulosa luteinized cells, modulating the activity of 3 beta-HSD. It is likely that these actions may be mediated via membrane receptors, possibly of the AT2 receptor family.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Progesterona / Progesterona Redutase / Angiotensina II / Receptores de Angiotensina / Células da Granulosa Limite: Female / Humans Idioma: En Revista: Mol Hum Reprod Assunto da revista: BIOLOGIA MOLECULAR / MEDICINA REPRODUTIVA Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Progesterona / Progesterona Redutase / Angiotensina II / Receptores de Angiotensina / Células da Granulosa Limite: Female / Humans Idioma: En Revista: Mol Hum Reprod Assunto da revista: BIOLOGIA MOLECULAR / MEDICINA REPRODUTIVA Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido