Action of antihistamine drugs in vitro-II. ion movements and phosphoproteins in whole cells

ABSTRACT

Slices of rat liver and suspensions of ascites cells were used to determine the effects of antihistamine drugs upon ion movements; it was shown that these drugs inhibit uptake of potassium in such systems. The relationship between phosphoproteins and these effects was also investigated, and there appeared to be a direct relationship between protein phosphorylation and water and ion movements; this relationship is not mediated by effects on ATP levels or turnover rates. The nature of the protein fraction that takes up 32P and shows diminished turnover in the presence of the drugs has been established by partial hydrolysis and identification of radioactive phosphoserine by chromatography on ion-exchange columns and upon paper(AU)