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Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein
Chek Meng Poh; Guillaume Carissimo; Wang Bei; Siti Naqiah Amrun; Cheryl Yi-Pin Lee; Rhonda Sin-Ling Chee; Nicholas Kim-Wah Yeo; Wen-Hsin Lee; Yee-Sin Leo; Mark I-Cheng Chen; Seow-Yen Tan; Louis Yi Ann Chai; Shirin Kalimuddin; Siew-Yee Thien; Barnaby Edward Young; David C. Lye; Cheng-I Wang; Laurent Renia; Lisa FP Ng.
Afiliação
  • Chek Meng Poh; Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, Singapore
  • Guillaume Carissimo; Singapore Immunology Network
  • Wang Bei; Singapore Immunology Network
  • Siti Naqiah Amrun; Singapore Immunology Network
  • Cheryl Yi-Pin Lee; Singapore Immunology Network
  • Rhonda Sin-Ling Chee; Singapore Immunology Network
  • Nicholas Kim-Wah Yeo; Singapore Immunology Network
  • Wen-Hsin Lee; Singapore Immunology Network
  • Yee-Sin Leo; National Centre for Infectious Diseases, Singapore
  • Mark I-Cheng Chen; National Centre for Infectious Diseases, Singapore
  • Seow-Yen Tan; Department of Infectious Diseases, Changi General Hospital, Singapore
  • Louis Yi Ann Chai; Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore
  • Shirin Kalimuddin; Department of Infectious Diseases, Singapore General Hospital, Singapore
  • Siew-Yee Thien; Department of Infectious Diseases, Singapore General Hospital, Singapore
  • Barnaby Edward Young; National Centre for Infectious Diseases, Singapore
  • David C. Lye; National Centre for Infectious Diseases, Singapore
  • Cheng-I Wang; Singapore Immunology Network
  • Laurent Renia; Singapore Immunology Network
  • Lisa FP Ng; Singapore Immunology Network
Preprint em En | PREPRINT-BIORXIV | ID: ppbiorxiv-015461
ABSTRACT
The ongoing SARS-CoV-2 pandemic demands rapid identification of immunogenic targets for the design of efficient vaccines and serological detection tools. In this report, using pools of overlapping linear peptides and functional assays, we present two immunodominant regions on the spike glycoprotein that were highly recognized by neutralizing antibodies in the sera of COVID-19 convalescent patients. One is highly specific to SARS-CoV-2, and the other is a potential pan-coronavirus target.
Licença
cc_no
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint