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New detection of SARS-CoV-2 in two cats height months after COVID-19 outbreak appearance in France
Matthieu Fritz; Nicolas Nesi; Solene Denolly; Bertrand Boson; Vincent Legros; Serge G. Rosolen; Alexandra Briend-Marchal; Meriadeg Ar Gouil; Eric M. Leroy.
Afiliação
  • Matthieu Fritz; Institut de Recherche pour le Developpement
  • Nicolas Nesi; Universite de Caen Normandie
  • Solene Denolly; Centre International de Recherche en Infectiologie
  • Bertrand Boson; Centre International de Recherche en Infectiologie
  • Vincent Legros; Centre International de Recherche en Infectiologie/VetAgro Sup
  • Serge G. Rosolen; Sorbonne Universite/Clinique Veterinaire Voltaire
  • Alexandra Briend-Marchal; Laboratoire de Biologie Veterinaire VEBIO
  • Meriadeg Ar Gouil; Universite de Caen Normandie/Centre Hospitalo-Universitaire Caen
  • Eric M. Leroy; Institut de Recherche pour le Developpement
Preprint em En | PREPRINT-BIORXIV | ID: ppbiorxiv-436830
ABSTRACT
Although there are several reports in the literature of SARS-CoV-2 infection in cats, few SARS-CoV-2 sequences from infected cats have been published. In this report, SARS-CoV-2 infection was evaluated in two cats by clinical observation, molecular biology (qPCR and NGS), and serology (Microsphere immunoassay and seroneutralization). Following the observation of symptomatic SARS-CoV-2-infection in two cats, infection status was confirmed by RT-qPCR and, in one cat, serological analysis for antibodies against N-protein and S-protein, as well as neutralizing antibodies. Comparative analysis of five SARS-CoV-2 sequence-fragments obtained from one of the cats showed that this infection was not with one of the three recently emerged variants of SARS-CoV-2. This study provides additional information on the clinical, molecular, and serological aspects of SARS-CoV-2 infection in cats.
Licença
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Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Tipo de estudo: Experimental_studies / Observational_studies / Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Preprint
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Tipo de estudo: Experimental_studies / Observational_studies / Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Preprint