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Amplicon-based nanopore minion sequencing of patients with COVID-19 omicron variant from India
Somesh Kumar; Avinash Lomash; Mohammed Faruq; Oves Siddiqui; Suresh Kumar; Seema v; Prashanth Suravajhala; Sunil K Polipalli; - SCOG_MAMC_ LNH.
Afiliação
  • Somesh Kumar; Genome Sequencing Laboratory, Lok Nayak Hospital, Jawaharlal Nehru Marg, Near Delhi Gate, New Delhi, Delhi 110002, India
  • Avinash Lomash; Genome Sequencing Laboratory, Lok Nayak Hospital, Jawaharlal Nehru Marg, Near Delhi Gate, New Delhi, Delhi 110002, India
  • Mohammed Faruq; CSIR-IGIB
  • Oves Siddiqui; Department of Microbiology,Maulana Azad Medical College,BSZ Marg, New Delhi-110002
  • Suresh Kumar; Genome Sequencing Laboratory, Lok Nayak Hospital, Jawaharlal Nehru Marg, Near Delhi Gate, New Delhi, Delhi 110002, India
  • Seema v; Genome Sequencing Laboratory, Lok Nayak Hospital, Jawaharlal Nehru Marg, Near Delhi Gate, New Delhi, Delhi 110002, India
  • Prashanth Suravajhala; Amrita University Kerala
  • Sunil K Polipalli; Genome Sequencing Laboratory, Lok Nayak Hospital, Jawaharlal Nehru Marg, Near Delhi Gate, New Delhi, Delhi 110002, India
  • - SCOG_MAMC_ LNH;
Preprint em Inglês | medRxiv | ID: ppmedrxiv-21268364
ABSTRACT
SARS-CoV-2 infection has been playing havoc with emerging omicron variants of concern (VoC). Here, we report sequencing of the omicron variant in 13 patients from India using Oxford Nanopore Technology (ONT) Minion, wherein a rapid amplicon based sequence analysis was performed to assess and compare with existing 34 mutations in spike glycoprotein. We highlight and discuss the nature of these mutations that are unique and common to other populations. This is perhaps the first report on omicron variants from India using a long read sequencing chemistry.
Licença
cc_by_nc
Texto completo: Disponível Coleções: Preprints Base de dados: medRxiv Idioma: Inglês Ano de publicação: 2021 Tipo de documento: Preprint
Texto completo: Disponível Coleções: Preprints Base de dados: medRxiv Idioma: Inglês Ano de publicação: 2021 Tipo de documento: Preprint
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