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Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models
Pimentel, Muriel Magda Lustosa; Santos, Fernanda Araujo dos; Macêdo, Luã Barbalho de; Brito, Parmênedes Dias de; Lima, Gabriela Liberalino; Barreto Junior, Raimundo Alves; Bezerra, Marcelo Barbosa.
Afiliação
  • Pimentel, Muriel Magda Lustosa; Centro Universitário Cesmac. Maceió. Brasil
  • Santos, Fernanda Araujo dos; Universidade Federal Rural do Semi-Árido. Mossoró. Brasil
  • Macêdo, Luã Barbalho de; Universidade Federal Rural do Semi-Árido. Mossoró. Brasil
  • Brito, Parmênedes Dias de; Universidade Federal Rural do Semi-Árido. Mossoró. Brasil
  • Lima, Gabriela Liberalino; Instituto Federal de Educação, Ciência e Tecnologia do Ceará. Departamento de Ciência Animal. Crato. Brasil
  • Barreto Junior, Raimundo Alves; Universidade Federal Rural do Semi-Árido. Departamento de Ciência Animal. Mossoró. Brasil
  • Bezerra, Marcelo Barbosa; Universidade Federal Rural do Semi-Árido. Departamento de Ciência Animal. Mossoró. Brasil
Anim. Reprod. ; 17(2): e20190115, 2020. ilus, tab
Article em En | VETINDEX | ID: vti-29308
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Ruminantes / Criopreservação / Vitrificação / Fase Folicular Limite: Animals Idioma: En Revista: Anim. Reprod. / Anim. Reprod. (Online) Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Ruminantes / Criopreservação / Vitrificação / Fase Folicular Limite: Animals Idioma: En Revista: Anim. Reprod. / Anim. Reprod. (Online) Ano de publicação: 2020 Tipo de documento: Article