Restoration of adenosine deaminase, histamine, and IgE in organs of mice injected with cobra venom followed by specific treatment and reversal period

ABSTRACT

Adenosine deaminase (ADA), histamine, and IgE are endogenously present in animals. Research from this laboratory reported decreased levels of these substances in organs of mice as a consequence of sub-lethal injection of Naja kaouthia venom. This research reports that decreased ADA, histamine, and IgE levels were prevented by specific treatment and prolonged recovery periods. Adult Balb/c mice injected IM with sub-lethal venom dose were divided into five groups. Group 1 were injected with PBS; Group 2 with anti-cobra venom; and Group 3 with lethal toxin neutralizing factor (LTNF). Groups 4 and 5 were treated with IM or oral synthetic LT-10. After 24 hours, mice were sacrificed and organ homogenates were assayed for ADA, histamine, and IgE. Group 1 showed substantial reduction in levels of these substances. It was revealed that decreased levels were prevented by treatment with anti-cobra venom, LTNF, and LT-10. In a second series of experiments, venom-injected mice were sacrificed after 3, 7, and 10 days and organs assayed for ADA, histamine, and IgE levels. The recovery period to homeostasis for ADA, histamine, and IgE was 7 to 10 days.