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Valores hematológicos e bioquímicos de camundongos imunossuprimidos BALB/c NUDE E C57BL/6 SCID / Haematological and biochemical values of immunosuppressed BALB/c NUDE and C57BL/6 SCID mice
Praxedes, Érika Almeida; Pimentel, Muriel Magda Lustosa; Santos, Fernanda Araujo dos; Fernandes, Denilsa Pires; Barbosa, Brenna de Sousa; Brito, Parmênedes Dias de; Lelis, Ivana Cristina Nunes Gadelha; Macedo, Michelly Fernandes de; Bezerra, Marcelo Barbosa.
Afiliação
  • Praxedes, Érika Almeida; Universidade Federal Rural do Semi-Árido. Mossoró. BR
  • Pimentel, Muriel Magda Lustosa; Universidade Federal Rural do Semi-Árido. Mossoró. BR
  • Santos, Fernanda Araujo dos; Universidade Federal Rural do Semi-Árido. Mossoró. BR
  • Fernandes, Denilsa Pires; Universidade Federal Rural do Semi-Árido. Mossoró. BR
  • Barbosa, Brenna de Sousa; Universidade Federal Rural do Semi-Árido. Mossoró. BR
  • Brito, Parmênedes Dias de; Universidade Federal Rural do Semi-Árido. Mossoró. BR
  • Lelis, Ivana Cristina Nunes Gadelha; Universidade Federal Rural do Semi-Árido. Mossoró. BR
  • Macedo, Michelly Fernandes de; Universidade Federal Rural do Semi-Árido. Mossoró. BR
  • Bezerra, Marcelo Barbosa; Universidade Federal Rural do Semi-Árido. Mossoró. BR
Acta sci. vet. (Impr.) ; 46: Pub.1603-2018. tab
Article em Pt | VETINDEX | ID: biblio-1457893
Biblioteca responsável: BR68.1
ABSTRACT

Background:

The emergence of the NUDE and SCID immunosuppressed mice lineages generated knowledge on various mechanisms of lymphocyte maturation and human autoimmune diseases. Information on haematological and biochemical parameters of these lineages is still scarce, making it impossible to infer homeostasis by comparing data, or to detect genetic influences on the parameters for these species. Haematological and biochemical tests were carried out on Balb/c NUDE and C57BL/6 SCID mice of both sexes, aiming to analyse the presence of genetic influence on possible variations of such parameters and to verify reference values for both lineages.Materials, Methods &

Results:

One hundred and forty mice (Mus musculus) of the Balb/C NUDE and C57BL/6 SCID lineages were used in the present study. The animals were previously anesthetized, the blood collection procedure was performed by cardiac puncture and the samples were collected in the presence of heparin and intended for haematological and biochemical evaluation, under standardized conditions. The haematological evaluation consisted of red blood cell count, leukocyte counts, platelet counts, haematocrit, haemoglobin concentration, mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC). The quantified biochemical parameters were urea, creatinine, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST) and alkaline phosphatase (ALP). While analysing the obtained data, it was possible to observe that only females presented divergences (P < 0.05) in the red blood cell series, in haemoglobin and in mean haemoglobin concentration (MCH). Regarding the analysis of the white blood cell series, females only presented differences (P < 0.05) in the leukocyte count. For males, there were variations (P < 0.05) in the counts of leukocytes, eosinophils, lymphocytes and neutrophils.[...]
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Variação Genética / Biomarcadores / Terapia de Imunossupressão Limite: Animals Idioma: Pt Revista: Acta sci. vet. (Impr.) / Acta sci. vet. (Online) Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Variação Genética / Biomarcadores / Terapia de Imunossupressão Limite: Animals Idioma: Pt Revista: Acta sci. vet. (Impr.) / Acta sci. vet. (Online) Ano de publicação: 2018 Tipo de documento: Article