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PCR and ELISA (VIDAS ECO O157(R)) Escherichia coli O157: H7 identification in Minas Frescal cheese commercialized in Goiânia, GO
Carvalho, Rosangela Nunes; Oliveira, Antonio Nonato de; Mesquita, Albenones José de; Rezende, Cíntia Silva Minafra e; Mesquita, Adriano Queiroz de; Romero, Rolando Alfredo Mazzoni.
Afiliação
  • Carvalho, Rosangela Nunes; Embrapa Arroz e Feijão. Goiás. BR
  • Oliveira, Antonio Nonato de; Embrapa Arroz e Feijão. Goiás. BR
  • Mesquita, Albenones José de; Embrapa Arroz e Feijão. Goiás. BR
  • Rezende, Cíntia Silva Minafra e; Embrapa Arroz e Feijão. Goiás. BR
  • Mesquita, Adriano Queiroz de; Embrapa Arroz e Feijão. Goiás. BR
  • Romero, Rolando Alfredo Mazzoni; Embrapa Arroz e Feijão. Goiás. BR
Braz. J. Microbiol. ; 45(1): 7-10, 2014. ilus
Article em En | VETINDEX | ID: vti-745914
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Escherichia coli O157H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Queijo / Reação em Cadeia da Polimerase Limite: Humans Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Queijo / Reação em Cadeia da Polimerase Limite: Humans Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2014 Tipo de documento: Article