Detection and characterization of verotoxin-producing strains among sorbitol non-fermenting Escherichia coli
(East. Mediterr. health j).
em Inglês
| WHO IRIS
| ID: who-119085
Biblioteca responsável:
CH1.1
ABSTRACT
The presence of genes for verotoxin 1 and 2 [VT1 and 2] among sorbitol non-fermenting Escherichia coli isolates from diarrhoeal cases was assessed using polymerase chain reaction assay. Of 60 [88%] positive isolates, 37 [62%] harboured VT1 and 23 [38%] both VT1 and VT2. In HeLa cell adherence assay, 48 [71%] isolates exhibited mannose-resistant adherence to HeLa cells. Multidrug resistance was observed in 56 [82%] isolates, with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim and tetracycline pattern being the most common. There were 13 common and 22 single biochemical phenotypes identified. Isolates belonging to common biochemical phenotypes normally had a similar pattern of adherence and VT production, but differed greatly in their pattern of antibiotic resistance, pointing to a high rate of antibiotic-resistance transfer among these isolates
Texto completo:
Disponível
Coleções:
Bases de dados de organismos internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Diarreia
/
Doenças Negligenciadas
/
Zoonoses
Base de dados:
WHO IRIS
Assunto principal:
Sorbitol
/
Aderência Bacteriana
/
DNA Bacteriano
/
Impressões Digitais de DNA
/
Técnicas de Cultura de Células
/
Escherichia coli O157
/
Toxina Shiga I
/
Toxina Shiga II
/
Farmacorresistência Bacteriana Múltipla
/
Diarreia
Idioma:
Inglês
Revista:
East. Mediterr. health j
Ano de publicação:
2001