Highly efficient production of L-valine by multiplex metabolic engineering of Corynebacterium glutamicum / 生物工程学报
Chinese Journal of Biotechnology
; (12): 3253-3272, 2023.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-1007956
Biblioteca responsável:
WPRO
ABSTRACT
As a branched chain amino acid, L-valine is widely used in the medicine and feed sectors. In this study, a microbial cell factory for efficient production of L-valine was constructed by combining various metabolic engineering strategies. First, precursor supply for L-valine biosynthesis was enhanced by strengthening the glycolysis pathway and weakening the metabolic pathway of by-products. Subsequently, the key enzyme in the L-valine synthesis pathway, acetylhydroxylate synthase, was engineered by site-directed mutation to relieve the feedback inhibition of the engineered strain. Moreover, promoter engineering was used to optimize the gene expression level of key enzymes in L-valine biosynthetic pathway. Furthermore, cofactor engineering was adopted to change the cofactor preference of acetohydroxyacid isomeroreductase and branched-chain amino acid aminotransferase from NADPH to NADH. The engineered strain C. glutamicum K020 showed a significant increase in L-valine titer, yield and productivity in 5 L fed-batch bioreactor, up to 110 g/L, 0.51 g/g and 2.29 g/(L‧h), respectively.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Valina
/
Reatores Biológicos
/
Corynebacterium glutamicum
/
Engenharia Metabólica
/
Aminoácidos de Cadeia Ramificada
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2023
Tipo de documento:
Artigo