Structural characterization of PCP-Ⅰ from Poria as vaccine adjuvant and its hydrolytic oligosaccharide / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4429-4437, 2023.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-1008697
Biblioteca responsável:
WPRO
ABSTRACT
Poria is an important medical herb in clinic. The authors isolated a polysaccharide(PCP-Ⅰ) from Poria in previous studies, which is composed of galactose, mannose, fucose and glucose. PCP-Ⅰ exhibited significant adjuvant effects on H1N1 influenza vaccine, hepatitis B surface antigen and anthrax protective antigen, and its adjuvant activity was stronger than aluminium adjuvant. However, little is known about the chemical structure of PCP-Ⅰ at present. In this study, weak acid hydrolysis was used to obtain the backbone oligosaccharide of PCP-Ⅰ. Then periodate oxidation, Smith degradation, methylation analysis, Fourier transform infrared spectroscopy(FT-IR), nuclear magnetic resonance(NMR) and gas chromatography-mass spectrometry(GC-MS) were performed to investigate the chemical structural features of PCP-Ⅰ and its hydrolytic oligosaccharide(PCP-Ⅰ-hy-1). These results suggested that the backbone of PCP-Ⅰ was composed of galactose with α anomeric carbon and β anomeric carbon. The linking residues of galactan are(1→),(l→6) and(1→2,6).
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligossacarídeos
/
Polissacarídeos
/
Carbono
/
Espectroscopia de Infravermelho com Transformada de Fourier
/
Vírus da Influenza A Subtipo H1N1
/
Poria
/
Galactose
/
Adjuvantes de Vacinas
/
Hidrólise
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2023
Tipo de documento:
Artigo